The overall goal of this grant is to elucidate the physiological mechanisms by which short-term changes in food intake lead to changes in the central neural drive to the reproductive axis in primate species. Brief periods of fasting (i.e., one to two days) lead to a significant suppression of LH, FSH and testosterone secretion in adult male rhesus monkeys and men. Subsequent refeeding of monkeys after a brief period of fasting leads to a rapid and dramatic increase in LH and testosterone secretion within the first hour after meal intake. Using the experimental paradigms of fasting and refeeding the applicant proposes to: 1) identify neuronal systems which may mediate the changes in LH secretion caused by fasting and refeeding. Experiments will examine the role of three specific neural systems in mediating nutrition-induced changes in LH secretion; the vagus nerves, noradrenergic pathways, and NPY-containing neurons. She further proposes to identify other central neural systems activated by fasting and refeeding by determining the transient expression of cFos and Fos related antigens (FRAs) in identified neuronal populations by using in situ hybridization and immunocytochemical techniques; 2) determine the role of insulin and T3 in causing the suppression of LH secretion during fasting and the restoration of LH secretion during refeeding. 3) determine the extent to which the diurnal pattern of LH secretion in the male rhesus monkey is determined by the pattern of daily food intake, and the timecourse with which changes in the pattern of food intake modify the pattern of LH secretion.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
2R01HD026888-04A3
Application #
2200133
Study Section
Reproductive Endocrinology Study Section (REN)
Project Start
1990-04-01
Project End
1999-04-30
Budget Start
1995-05-01
Budget End
1996-04-30
Support Year
4
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of Pittsburgh
Department
Psychiatry
Type
Schools of Medicine
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Grimes, Michael A; Cameron, Judy L; Fernstrom, John D (2009) Cerebrospinal fluid concentrations of large neutral and basic amino acids in Macaca mulatta: diurnal variations and responses to chronic changes in dietary protein intake. Metabolism 58:129-40
Mancini, Fulvia; Loucks, Tammy L; Cameron, Judy L et al. (2005) Sex steroid milieu does not alter the impact of fasting on leptin levels in women. Fertil Steril 84:1768-71
Cameron, Judy L (2004) Interrelationships between hormones, behavior, and affect during adolescence: complex relationships exist between reproductive hormones, stress-related hormones, and the activity of neural systems that regulate behavioral affect. Comments on part III. Ann N Y Acad Sci 1021:134-42
Berga, S L; Loucks, T L; Cameron, J L (2001) Endocrine and chronobiological effects of fasting in women. Fertil Steril 75:926-32
Grimes, M A; Cameron, J L; Fernstrom, J D (2000) Cerebrospinal fluid concentrations of tryptophan and 5-hydroxyindoleacetic acid in Macaca mulatta: diurnal variations and response to chronic changes in dietary protein intake. Neurochem Res 25:413-22
Tang-Christensen, M; Havel, P J; Jacobs, R R et al. (1999) Central administration of leptin inhibits food intake and activates the sympathetic nervous system in rhesus macaques. J Clin Endocrinol Metab 84:711-7
Larsen, P J; Tang-Christensen, M; Stidsen, C E et al. (1999) Activation of central neuropeptide Y Y1 receptors potently stimulates food intake in male rhesus monkeys. J Clin Endocrinol Metab 84:3781-91
Caston-Balderrama, A L; Cameron, J L; Hoffman, G E (1998) Immunocytochemical localization of Fos in perfused nonhuman primate brain tissue: fixation and antisera selection. J Histochem Cytochem 46:547-56
Schreihofer, D A; Cameron, J L; Verbalis, J G et al. (1997) Cholecystokinin induces Fos expression in catecholaminergic neurons of the macaque monkey caudal medulla. Brain Res 770:37-44
Cameron, J L (1996) Nutritional determinants of puberty. Nutr Rev 54:S17-22

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