Abstract

This application will test the hypothesis that during parturition glutamine is diverted from fetal hepatic glutamate output to glycogen synthesis. The proposed studies will examine by multiple tracer methodology the interrelationship between fetal hepatic glucose production and glutamine metabolism and glutamate release during spontaneous parturition, and during the stimulation of fetal hepatic gluconeogenesis by cortisol. Other experiments will examine the metabolic and physiologic impact of maintaining a high fetal glutamate supply to the placenta around the time of parturition.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD029374-06
Application #
2673685
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Project Start
1992-08-01
Project End
1999-07-31
Budget Start
1998-08-01
Budget End
1999-07-31
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Physiology
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Timmerman, M; Wilkening, R B; Regnault, T R H (2003) Induction of glutamate dehydrogenase in the ovine fetal liver by dexamethasone infusion during late gestation. Exp Biol Med (Maywood) 228:100-5
Timmerman, M; Teng, C; Wilkening, R B et al. (2001) Net amino acid flux across the fetal liver and placenta during spontaneous ovine parturition. Biol Neonate 79:54-60
Timmerman, M; Teng, C; Wilkening, R B et al. (2000) Effect of dexamethasone on fetal hepatic glutamine-glutamate exchange. Am J Physiol Endocrinol Metab 278:E839-45
Jozwik, M; Teng, C; Meschia, G et al. (1999) Contribution of branched-chain amino acids to uteroplacental ammonia production in sheep. Biol Reprod 61:792-6
Holcomb, R G; Wilkening, R B (1999) Fetal hepatic oxygen consumption under normal conditions in the fetal lamb. Biol Neonate 75:310-8
Jozwik, M; Teng, C; Battaglia, F C et al. (1999) Fetal supply of amino acids and amino nitrogen after maternal infusion of amino acids in pregnant sheep. Am J Obstet Gynecol 180:447-53
Chung, M; Teng, C; Timmerman, M et al. (1998) Production and utilization of amino acids by ovine placenta in vivo. Am J Physiol 274:E13-22
Barbera, A; Wilkening, R B; Teng, C et al. (1997) Metabolic alterations in the fetal hepatic and umbilical circulations during glucocorticoid-induced parturition in sheep. Pediatr Res 41:242-8
Vaughn, P R; Lobo, C; Battaglia, F C et al. (1995) Glutamine-glutamate exchange between placenta and fetal liver. Am J Physiol 268:E705-11
Moores Jr, R R; Vaughn, P R; Battaglia, F C et al. (1994) Glutamate metabolism in fetus and placenta of late-gestation sheep. Am J Physiol 267:R89-96