The studies proposed in this application will: (1) determine if progesterone withdrawal at different stages of decidualization would affect the expression of bcl-2 gene family members; this information will be correlated with DNA fragmentation in the decidual cells; (2) investigate why induced Bax expression failed to cause death of decidual cells by determining if Bax activity is compromised by Bcl-xL or hsp-25; (3) determine the expression of TGF-beta and apoptotic mediators (cathepsin-D, cytochrome- c, apaf-1, and caspase-3) in the GG-AD decidual cell line, and assess the consequence of overexpressing bax or inhibiting bax and cathepsin-D in GG-AD cells.
| Ingraham, Susan E; Lynch, Roy A; Surti, Urvashi et al. (2006) Identification and characterization of novel human transcripts embedded within HMGA2 in t(12;14)(q15;q24.1) uterine leiomyoma. Mutat Res 602:43-53 |
| Akcali, Kamil C; Gibori, Geula; Khan, Sohaib A (2003) The involvement of apoptotic regulators during in vitro decidualization. Eur J Endocrinol 149:69-75 |
| Dai, D; Moulton, B C; Ogle, T F (2000) Regression of the decidualized mesometrium and decidual cell apoptosis are associated with a shift in expression of Bcl2 family members. Biol Reprod 63:188-95 |
| Akcali, K C; Khan, S A; Moulton, B C (1996) Effect of decidualization on the expression of bax and bcl-2 in the rat uterine endometrium. Endocrinology 137:3123-31 |
