Sperm Ion Channel Regulation

Florman, Harvey M.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
• Type: Research Project (R01)
• Project #: 7R01HD032177-03
• Application #: 2205163
• Study Section: Reproductive Biology Study Section (REB)
• Project Start: 1994-08-01
• Project End: 1998-05-31
• Budget Start: 1996-01-01
• Budget End: 1996-05-31
• Support Year: 3
• Fiscal Year: 1995

Institution

• Name: Tufts University
• Department: Anatomy/Cell Biology
• Type: Schools of Medicine
• DUNS #: 604483045
• City: Boston
• State: MA
• Country: United States
• Zip Code: 02111

Publications

Galantino-Homer, Hannah L; Florman, Harvey M; Storey, Bayard T et al. (2004) Bovine sperm capacitation: assessment of phosphodiesterase activity and intracellular alkalinization on capacitation-associated protein tyrosine phosphorylation. Mol Reprod Dev 67:487-500

Lopez-Gonzalez, I; De La Vega-Beltran, J L; Santi, C M et al. (2001) Calmodulin antagonists inhibit T-type Ca(2+) currents in mouse spermatogenic cells and the zona pellucida-induced sperm acrosome reaction. Dev Biol 236:210-9

Gonzalez-Martinez, M T; Galindo, B E; de De La Torre, L et al. (2001) A sustained increase in intracellular Ca(2+) is required for the acrosome reaction in sea urchin sperm. Dev Biol 236:220-9

O'Toole, C M; Arnoult, C; Darszon, A et al. (2000) Ca(2+) entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction. Mol Biol Cell 11:1571-84

Arnoult, C; Kazam, I G; Visconti, P E et al. (1999) Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation. Proc Natl Acad Sci U S A 96:6757-62

Arnoult, C; Villaz, M; Florman, H M (1998) Pharmacological properties of the T-type Ca2+ current of mouse spermatogenic cells. Mol Pharmacol 53:1104-11

Florman, H M; Arnoult, C; Kazam, I G et al. (1998) A perspective on the control of mammalian fertilization by egg-activated ion channels in sperm: a tale of two channels. Biol Reprod 59:12-6

Arnoult, C; Cardullo, R A; Lemos, J R et al. (1996) Activation of mouse sperm T-type Ca2+ channels by adhesion to the egg zona pellucida. Proc Natl Acad Sci U S A 93:13004-9

Arnoult, C; Zeng, Y; Florman, H M (1996) ZP3-dependent activation of sperm cation channels regulates acrosomal secretion during mammalian fertilization. J Cell Biol 134:637-45

Zeng, Y; Clark, E N; Florman, H M (1995) Sperm membrane potential: hyperpolarization during capacitation regulates zona pellucida-dependent acrosomal secretion. Dev Biol 171:554-63