Based on the hypothesis that activated E receptor (ER) functions via association with one or more transcription factors that bind directly to -105/-72 DNA, the following aims are proposed:
Aim 1 seeks to define the DNA sequences necessary for repression of FSH beta by E;
Aim 2 will identify the nuclear proteins that bind directly to the -105 to -72 bp region and determine how they interact with the activated ER;
Aim 3 will use existing mutants of the ER to define the characteristics necessary for repression;
and Aim 4 involves the production of transgenic mice that contain either wild-type or mutated ovine FSH beta genes to determine which binding sites in the -105/-72 sequence are critical for negative regulation in vivo.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
3R01HD034863-03S1
Application #
6142982
Study Section
Biochemical Endocrinology Study Section (BCE)
Program Officer
De Paolo, Louis V
Project Start
1996-08-06
Project End
2000-12-31
Budget Start
1998-07-01
Budget End
2000-12-31
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
North Carolina State University Raleigh
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
City
Raleigh
State
NC
Country
United States
Zip Code
27695