A proper dialogue between the conceptus and mother is critical for the successful establishment of normal pregnancy. Our long-range goal is to identify and determine the function of genes whose expression in the endometrium is controlled by the conceptus after the onset of implantation as the endometrium undergoes decidualization. The proposed hypothesis is that there are conceptus-dependent changes in the endometrium during the days after the onset of implantation. These changes include gene expression, angiogenesis/vascular remodeling and immune cell numbers.
Specific aims are to determine in the mouse that: (1) there are conceptus-dependent changes in the endometrial expression of """"""""angio-modulatory"""""""" genes during implantation, (2) there are conceptus-dependent changes in the endometrial vasculature during implantation and (3) uterine natural killer cells are mediators of conceptus-dependant vascular changes in the endometrium during implantation. Several in vivo approaches will be used including using normal and """"""""knockout"""""""" mice and mice undergoing artificially-induced decidualization. In vitro approaches will involve the use of trophoblast stem cell, giant cell and endometrial stromal cell cultures. Basic research to better understand how the conceptus influences the changes that occur in the endometrium just after the onset of implantation may provide insights that can be used to develop: treatments for infertility, methods to prevent later gestational problems, or new and improved contraceptive methods. ? ?

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD049010-04
Application #
7420902
Study Section
Pregnancy and Neonatology Study Section (PN)
Program Officer
Yoshinaga, Koji
Project Start
2005-07-01
Project End
2010-04-30
Budget Start
2008-05-01
Budget End
2009-04-30
Support Year
4
Fiscal Year
2008
Total Cost
$199,729
Indirect Cost
Name
Southern Illinois University Carbondale
Department
Physiology
Type
Schools of Medicine
DUNS #
939007555
City
Carbondale
State
IL
Country
United States
Zip Code
62901
Scott, Charles A; Eckstrum, Kirsten S; Bany, Brent M (2012) Localization of C-Fos-Induced Growth Factor (Figf) mRNA Expression in the Mouse Uterus during Implantation. Reprod Syst Sex Disord Suppl 1:003
Scott, Charles A; van Huyen, Doan; Bany, Brent M (2012) Angiopoietin-like gene expression in the mouse uterus during implantation and in response to steroids. Cell Tissue Res 348:199-211
Bany, Brent M; Scott, Charles A; Eckstrum, Kirsten S (2012) Analysis of uterine gene expression in interleukin-15 knockout mice reveals uterine natural killer cells do not play a major role in decidualization and associated angiogenesis. Reproduction 143:359-75
McConaha, M E; Eckstrum, K; An, J et al. (2011) Microarray assessment of the influence of the conceptus on gene expression in the mouse uterus during decidualization. Reproduction 141:511-27
Huyen, D V; Bany, B M (2011) Evidence for a conserved function of heart and neural crest derivatives expressed transcript 2 in mouse and human decidualization. Reproduction 142:353-68
Eckstrum, Kirsten; Bany, Brent M (2011) Tumor necrosis factor receptor subfamily 9 (Tnfrsf9) gene is expressed in distinct cell populations in mouse uterus and conceptus during implantation period of pregnancy. Cell Tissue Res 344:567-76
Bany, Brent M; Hamilton, G Scot (2011) Assessment of permeability barriers to macromolecules in the rodent endometrium at the onset of implantation. Methods Mol Biol 763:83-94
Herington, Jennifer L; Bany, Brent M (2009) Do molecular signals from the conceptus influence endometrium decidualization in rodents? J Exp Zool B Mol Dev Evol 312:797-816
Herington, Jennifer L; Underwood, Tawny; McConaha, Melinda et al. (2009) Paracrine signals from the mouse conceptus are not required for the normal progression of decidualization. Endocrinology 150:4404-13
Herington, Jennifer L; Bany, Brent M (2007) The conceptus increases secreted phosphoprotein 1 gene expression in the mouse uterus during the progression of decidualization mainly due to its effects on uterine natural killer cells. Reproduction 133:1213-21

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