The overall objective of this study is to develop a resource for high resolution mapping, initially for markers on chromosomes 17 and X; and subsequently, for other chromosomes. The resource will be developed by identifying meiotic fragments that are defined by crossovers in 60 CEPH families. The meiotic mapping panel will greatly improve the efficiency and reliability of genetic mapping. The potential resolution of the CEPH resource is about 0.01 cM for autosomes and 0.02 cM for chromosome X. In order to develop the mapping panel resource most efficiently, it will be necessary to modify and extend existing computer programs that are currently used for haplotyping and quality control of CEPH marker data. The current density of markers for chromosomes 17 and X is sufficient to identify nearly all crossovers unambiguously using these simple algorithms. The position of each crossover will be validated by duplicate typing of the nearest flanking markers. Additional meioses will be screened for recombinants between tightly linked loci that cannot be ordered by other means. Selective typing of additional markers will be done in a directed effort to close the gap surrounding each crossover. This strategy will improve resolution and, at the same time, refine the localization of the new marker. Computer algorithms for translating genetic data into a physical mapping representation will be developed as a simple means of combining and comparing the results of genetic and physical mapping studies.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
5R01HG000360-06
Application #
2208778
Study Section
Mammalian Genetics Study Section (MGN)
Project Start
1988-09-28
Project End
1995-08-31
Budget Start
1993-09-01
Budget End
1995-08-31
Support Year
6
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Utah
Department
Biostatistics & Other Math Sci
Type
Schools of Medicine
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Fain, P R; Kort, E N; Yousry, C et al. (1996) A high resolution CEPH crossover mapping panel and integrated map of chromosome 11. Hum Mol Genet 5:1631-6
Fain, P R; Kort, E N; Chance, P F et al. (1995) A 2D crossover-based map of the human X chromosome as a model for map integration. Nat Genet 9:261-6
Litt, M; Kramer, P; Kort, E et al. (1995) The CEPH consortium linkage map of human chromosome 11. Genomics 27:101-12
Fain, P R; Barker, D F; Chance, P F (1994) Refined genetic mapping of X-linked Charcot-Marie-Tooth neuropathy. Am J Hum Genet 54:229-35
Barker, D F; Cordray, P; Fain, P R (1994) The same polymorphism identified by the DXS571(B) and DXS1105 loci. Hum Mol Genet 3:1913
Barker, D F; Nguyen, K; Fain, P R (1993) A CA dinucleotide polymorphism at D17S107 (17q12-q24). Hum Mol Genet 2:1086
Econs, M J; Fain, P R; Norman, M et al. (1993) Flanking markers define the X-linked hypophosphatemic rickets gene locus. J Bone Miner Res 8:1149-52
Barker, D F; Nguyen, K; Fain, P R (1993) Two simple repeat polymorphisms at DXS337. Hum Mol Genet 2:1507
Barker, D F; Fain, P R (1993) Definition and mapping of STSs at STR and RFLP loci in Xp11-Xq22. Genomics 18:712-6
Barker, D F; Nguyen, K; Fain, P R (1992) A CA-dinucleotide polymorphism at the D17S113 locus, which is closely linked to D17S74. Nucleic Acids Res 20:923

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