Abstract

investigator's Specific Aims) In this renewal application, Dr. Soares proposes to introduce some minor modifications in his protocol to construct libraries enriched for full-length cDNAs. Second, he will construct normalized libraries enriched for full-length cDNAs from size fractionated mRNAs representing a variety of human fetal and adult tissues. And third, cDNAs will be directionally cloned into an expression vector (pcDNA3.1/Zeo) which allows for high-level, stable or transient expression in a variety of mouse embryonic and adult tissues.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
5R01HG000980-06
Application #
2889655
Study Section
Special Emphasis Panel (ZRG2-BIOL-1 (01))
Program Officer
Feingold, Elise A
Project Start
1994-06-10
Project End
2001-06-30
Budget Start
1999-07-01
Budget End
2001-06-30
Support Year
6
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Pediatrics
Type
Schools of Medicine
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Lawton, L N; Bonaldo, M F; Jelenc, P C et al. (1997) Identification of a novel member of the TGF-beta superfamily highly expressed in human placenta. Gene 203:17-26
Bonaldo, M F; Lennon, G; Soares, M B (1996) Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res 6:791-806
Yoon, S J; LeBlanc-Straceski, J; Ward, D et al. (1994) Organization of the human keratin type II gene cluster at 12q13. Genomics 24:502-8