1) Studies on the mode of action of SPF, the rat liver protein which stimulates the microsome-associated transformation of squalene to squalene epoxide and of squalene epoxide to lanosterol. 2) Examination of sterol structure function relationships with model membranes, with the sterol-requiring bacterium Mycoplasma capricolum and the yeast sterol auxotroph GL7. 3) Studies on dual sterol functions in membranes with emphasis on the role of cholesterol in the control of phospholipid biosynthesis. 4) The functional significance of side-chain alkylation in the biosynthesis of yeast and plant sterols. The role of sterols in respiratory adaptation of yeast.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL002477-31
Application #
3334138
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1974-09-01
Project End
1988-02-29
Budget Start
1986-09-01
Budget End
1988-02-29
Support Year
31
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Harvard University
Department
Type
Schools of Arts and Sciences
DUNS #
071723621
City
Cambridge
State
MA
Country
United States
Zip Code
02138
Dahl, C; Biemann, H P; Dahl, J (1987) A protein kinase antigenically related to pp60v-src possibly involved in yeast cell cycle control: positive in vivo regulation by sterol. Proc Natl Acad Sci U S A 84:4012-6
Dahl, J S; Dahl, C E (1985) Stimulation of cell proliferation and polyphosphoinositide metabolism in Saccharomyces cerevisiae GL7 by ergosterol. Biochem Biophys Res Commun 133:844-50
Chin, J; Bloch, K (1985) Stimulation by unsaturated fatty acid of squalene uptake in rat liver microsomes. J Lipid Res 26:819-23
Dahl, C E; Sacktor, N C; Dahl, J S (1985) Acylated proteins in Acholeplasma laidlawii. J Bacteriol 162:445-7
Plank, L; Dahl, C E; Ware, B R (1985) Effect of sterol incorporation on head group separation in liposomes. Chem Phys Lipids 36:319-28