Abstract

The goal of these experiments is to characterize the reactions which constitute the contact phase of intrinsic clotting. They are designed to establish and define at the molecular level the intermediate reactions which take place in purified systems and in plasma between contact activators and factor XI, factor XII, high molecular weight kininogen (contact activation cofactor, Fitzgerald factor), and prekallikrein (Fletcher factor), which lead to the formation of activated factor XI. For these experiments highly purified clotting factors are prepared and radiolabelled with 125I. The reaction of contact activators and different combinations of purified reactants in isolated systems will be evaluated and compared to reaction mechanisms and products generated in plasma.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL017402-14
Application #
3335350
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1987-09-01
Project End
1992-08-31
Budget Start
1989-09-01
Budget End
1992-08-31
Support Year
14
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Southern California
Department
Type
Schools of Medicine
DUNS #
041544081
City
Los Angeles
State
CA
Country
United States
Zip Code
90033