Abstract

The overally objective is to examine the role of monoclonal cell populations in the origin and growth of atherosclerotic lesions in experimental animale and in man. Human studies will use the X-linked enzyme, Glucose-6-Phosphate Dehydrogenase, as a cellular marker for identification of monoclonal cell populations. The expression of certain genes associated with tumors will be examined in normal arterial wall, fatty streaks and atherosclerotic plaques as a test of the mutational theory of atherosclerosis. Intimal surfaces of aortas will be mapped to identify gross morphologic, histologic and histochemical characteristics of normal appearing intima and intimal lesions which have monoclonal characteristics. Animal studies will seek to further describe the hybrid hare as a promising model of human atherosclerosis. The lesions will be further studied for their clonal characteristics as well as histologic and histochemical features. Various dietary regimens will be employed to test the clonal selection theory. An immunofluorecence method for assessing isoenzyme characteristics in tissue sections will be developed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
2R01HL018473-12
Application #
3335594
Study Section
Pathology A Study Section (PTHA)
Project Start
1978-09-01
Project End
1991-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218