Abstract

A program of research concerned with definition of the biochemical process of protein degradation in mammalian cardiac tissue and investigation of the cellular regulatory mechanisms controlling this process is presented. A broad based approach utilizing in vitro techniques with intact tissue preparations (the isolated rabbit right ventricular papillary muscle), broken cell preparations (subcellular fractions isolated by differential centrifugation) and purified enzyme preparations (cathepsin-D) isolated from heart tissue is outlined. Particular effort has been given the development of improved methodology to allow for the investigation of specific problems not previously studied in mammalian myocardium.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL019648-10
Application #
3335881
Study Section
Cardiovascular and Pulmonary Research B Study Section (CVB)
Project Start
1976-07-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Northwestern University at Chicago
Department
Type
Schools of Medicine
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611

  Publications

Clark, W A; Rudnick, S J; Andersen, L C et al. (1994) Myosin heavy chain synthesis is independently regulated in hypertrophy and atrophy of isolated adult cardiac myocytes. J Biol Chem 269:25562-9
Clark, W A; Rudnick, S J; LaPres, J J et al. (1993) Regulation of hypertrophy and atrophy in cultured adult heart cells. Circ Res 73:1163-76
Decker, M L; Behnke-Barclay, M; Cook, M G et al. (1991) Cell shape and organization of the contractile apparatus in cultured adult cardiac myocytes. J Mol Cell Cardiol 23:817-32
Clark, W A; Rudnick, S J; LaPres, J J et al. (1991) Hypertrophy of isolated adult feline heart cells following beta-adrenergic-induced beating. Am J Physiol 261:C530-42
Decker, M L; Behnke-Barclay, M; Cook, M G et al. (1991) Morphometric evaluation of the contractile apparatus in primary cultures of rabbit cardiac myocytes. Circ Res 69:86-94
Decker, M L; Simpson, D G; Behnke, M et al. (1990) Morphological analysis of contracting and quiescent adult rabbit cardiac myocytes in long-term culture. Anat Rec 227:285-99
Samarel, A M; Ferguson, A G; Decker, R S et al. (1989) Effects of cysteine protease inhibitors on rabbit cathepsin D maturation. Am J Physiol 257:C1069-79
Coleman, P S; Parmacek, M S; Lesch, M et al. (1989) Protein synthesis and degradation during regression of thyroxine-induced cardiac hypertrophy. J Mol Cell Cardiol 21:911-25
Haddad, J; Decker, M L; Hsieh, L C et al. (1988) Attachment and maintenance of adult rabbit cardiac myocytes in primary cell culture. Am J Physiol 255:C19-27
Mostow, W R; Ferguson, A G; Lesch, M et al. (1988) Nonrandom turnover of actin and tubulin in cultured rabbit cardiac fibroblasts. Am J Physiol 255:C202-13

Showing the most recent 10 out of 18 publications