Abstract

We will study the interaction of desferrioxamine with iron ligands in reticulocytes, utilizing radioactive iron 59 as a probe for iron movement. Reticulocytes will be incubated in iron 59 labelled transferrin, hemolyzed, subjected to gel filtration on molecular sieve resins and radioactive fractions will be identified. These will be further fractionated utilizing electrophoresis and ion exchange chromatography.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL021560-06
Application #
3336569
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1978-12-01
Project End
1986-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Weaver, J; Zhan, H; Pollack, S (1993) Erythrocyte haemolysate interacts with ATP-Fe to form a complex containing iron, ATP and 13 800 MW polypeptide. Br J Haematol 83:138-44
Pollack, S (1992) Receptor-mediated iron uptake and intracellular iron transport. Am J Hematol 39:113-8
Zhan, H; Gupta, R K; Weaver, J et al. (1990) Iron bound to low MW ligands: interactions with mitochondria and cytosolic proteins. Eur J Haematol 44:125-31
Weaver, J; Pollack, S (1990) Iron binding to apotransferrin. Acta Haematol 84:68-71
Weaver, J; Zhan, H; Pollack, S (1990) Mitochondria have Fe(III) receptors. Biochem J 265:415-9
Jelicks, L A; Weaver, J; Pollack, S et al. (1989) NMR studies of intracellular free calcium, free magnesium and sodium in the guinea pig reticulocyte and mature red cell. Biochim Biophys Acta 1012:261-6
Zhan, H; Pollack, S; Weaver, J (1989) Hemolysates reduce iron released from transferrin. Am J Hematol 31:203-7
Pollack, S (1989) P. falciparum iron metabolism. Prog Clin Biol Res 313:151-61
Weaver, J; Pollack, S; Zhan, H (1989) Low molecular weight iron from guinea pig reticulocytes isolated by Sephadex G-25 chromatography. Eur J Haematol 43:321-7
Pollack, S; Schnelle, V (1988) Inability to detect transferrin receptors on P. falciparum parasitized red cells. Br J Haematol 68:125-9

Showing the most recent 10 out of 14 publications