Abstract

Three series of investigations are proposed. The first involves a comprehensive anatomical and physiological examination of the serotonergic raphe-SPN pathway. Using autoradiography, horseradish peroxidase (HRP), lesion, microstimulation, and extracellular recording techniques, studies have been designed to: (a) localize the cells of origin and spinal trajectory of the raphe-SPN system; (b) establish the conduction velocities and degree of convergence and divergence of raphe axons terminating upon the SPNs; (c) investigate the spatial and temporal properties of the inhibition of SPN discharge associated with activation of this descending system; (d) assess the involvement of the raphe in baroreceptor and somatosympathetic reflexes; and (e) establish the role of the raphe in the """"""""tonic"""""""" and """"""""phasic"""""""" modulation of arterial blood pressure. The second involves anterograde transport studies to localize the cells of origin of the catecholaminergic input(s) upon the SPNs. Using autoradiography, the spinal projections of cells in the ventrolateral medullary region, locus coeruleus, and subcoeruleus would be described. Anterograde tract tracing would also be initiated on the paraventricular nucleus of the hypothalamus. The final project involves a preliminary electron microscopic description of the cytological characteristics of axon terminals on HRP-labelled preganglionic neurons.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL024103-13
Application #
3337519
Study Section
Cardiovascular and Renal Study Section (CVB)
Project Start
1987-09-01
Project End
1992-08-31
Budget Start
1991-09-01
Budget End
1992-08-31
Support Year
13
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Type
Schools of Arts and Sciences
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Shao, Yufang; Akmentin, Wendy; Toledo-Aral, Juan Jose et al. (2002) Pincher, a pinocytic chaperone for nerve growth factor/TrkA signaling endosomes. J Cell Biol 157:679-91
Ligorio, M A; Akmentin, W; Gallery, F et al. (2000) Ultrastructural localization of the binding fragment of tetanus toxin in putative gamma-aminobutyric acidergic terminals in the intermediolateral cell column: a potential basis for sympathetic dysfunction in generalized tetanus. J Comp Neurol 419:471-84
Cabot, J B (1996) Some principles of the spinal organization of the sympathetic preganglionic outflow. Prog Brain Res 107:29-42
Cabot, J B; Bushnell, A; Alessi, V et al. (1995) Postsynaptic gephyrin immunoreactivity exhibits a nearly one-to-one correspondence with gamma-aminobutyric acid-like immunogold-labeled synaptic inputs to sympathetic preganglionic neurons. J Comp Neurol 356:418-32
Cabot, J B; Alessi, V; Carroll, J et al. (1994) Spinal cord lamina V and lamina VII interneuronal projections to sympathetic preganglionic neurons. J Comp Neurol 347:515-30
Cabot, J B; Alessi, V; Bushnell, A (1992) Glycine-like immunoreactive input to sympathetic preganglionic neurons. Brain Res 571:1-18
Cabot, J B; Mennone, A; Bogan, N et al. (1991) Retrograde, trans-synaptic and transneuronal transport of fragment C of tetanus toxin by sympathetic preganglionic neurons. Neuroscience 40:805-23
Bogan, N; Cabot, J B (1991) Light and electron microscopic analyses of intraspinal axon collaterals of sympathetic preganglionic neurons. Brain Res 541:241-51
Cabot, J B; Carroll, J; Bogan, N (1991) Localization of cardiac parasympathetic preganglionic neurons in the medulla oblongata of pigeon, Columba livia: a study using fragment C of tetanus toxin. Brain Res 544:162-8
Bogan, N; Mennone, A; Cabot, J B (1989) Light microscopic and ultrastructural localization of GABA-like immunoreactive input to retrogradely labeled sympathetic preganglionic neurons. Brain Res 505:257-70

Showing the most recent 10 out of 12 publications