Abstract

The physiological role of platelet membrane neutral glycolipids and gangliosides will be investigated by the analysis of the effects of specific anti-glycolipid antibodies on platelet function. The consequences of increasing the content of specific platelet plasma membrane gangliosides by the incorporation of exogenously supplied gangliosides also will be investigated. The effects of these alterations and modifications of platelet membrane glycolipids will be assessed on platelet functions, e.g., adhesion to collagen, aggregation, the release reaction, as well as on serotonin and alpha adrenergic receptor binding in platelets. The synthesis of glycolipids in platelets will be investigated. Also, the effects of thrombin on glycolipids and ganglioside synthesis in platelets will be investigated. Platelets from patients with disorders in which there is a high probability of abnormal platelet membrane glycolipids, e.g., myeloproliferative disorders, type IIa hyperlipidemia, and the Bernard Soulier syndrome, will be investigated. Platelets from these individuals will be evaluated for abnormal ganglioside and neutral glycolipid composition and structure. The fatty acid, sphingosine, and carbohydrate components of these glycolipids will be analyzed by gas-liquid chromatography.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL025455-07
Application #
3338082
Study Section
(EH)
Project Start
1979-08-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Temple University
Department
Type
Schools of Medicine
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19122

  Publications

Schick, P K; Walker, J; Profeta, B et al. (1997) Synthesis and secretion of von Willebrand factor and fibronectin in megakaryocytes at different phases of maturation. Arterioscler Thromb Vasc Biol 17:797-801
Schick, P K; Wojenski, C M; Bennett, V D et al. (1996) The synthesis and localization of alternatively spliced fibronectin EIIIB in resting and thrombin-treated megakaryocytes. Blood 87:1817-23
Schick, P K; Wojensk, C M; Bennett, V et al. (1996) Fibronectin isoforms in megakaryocytes. Stem Cells 14 Suppl 1:212-9
Schick, P K; Walker, J (1996) The acylation of megakaryocyte proteins: glycoprotein IX is primarily myristoylated while glycoprotein Ib is palmitoylated. Blood 87:1377-84
He, X; Schick, P K; Wojenski, C (1995) The expression of acetyl coenzyme A carboxylase is related to megakaryocyte maturation. J Lab Clin Med 126:178-83
Konkle, B A; Schick, P K; He, X et al. (1993) Plasminogen activator inhibitor-1 mRNA is expressed in platelets and megakaryocytes and the megakaryoblastic cell line CHRF-288. Arterioscler Thromb 13:669-74
Schick, P K; Wojenski, C M; Walker, J (1993) The effects of olive oil, hydrogenated palm oil, and omega-3 fatty acid-enriched diets on megakaryocytes and platelets. Arterioscler Thromb 13:84-9
Wojenski, C M; Schick, P K (1993) Development of storage granules during megakaryocyte maturation: accumulation of adenine nucleotides and the capacity for serotonin sequestration. J Lab Clin Med 121:479-85
Schick, P K; Konkle, B A; He, X et al. (1993) P-selectin mRNA is expressed at a later phase of megakaryocyte maturation than mRNAs for von Willebrand factor and glycoprotein Ib-alpha. J Lab Clin Med 121:714-21
Schick, P K; Williams-Gartner, K; He, X L (1990) Lipid composition and metabolism in megakaryocytes at different stages of maturation. J Lipid Res 31:27-35

Showing the most recent 10 out of 16 publications