I propose to carry out X-ray crystallographic studies on two transition state analoques of haemoglobin. One cross-linked in the oxy or R structure so that it maintains this structure even when deoxygenated, the other cross-linked in the deoxy or T structure which should maintain this structure at low temperature, even when liganded. These analyses should be done at a resolution sufficient to refine the structures and obtain atomic coordinates, so as to be able to see the changes in conformation of the haems and the surrounding globin, and the possible transimission of such changes to the subunit boundaries. I also propose to work on species adaptation in the haemoglobin molecule. Biochemists and physiologists have discovered cooperative binding of oxygen to be the only property that is common to all vertebrate haemoglobins, while their response to other ligands is subject to wide variations, determined by the animals' environment and habits. By working out the stereochemical mechanism of several such adaptive mechanisms I discovered that responses to different chemical stimuli can evolve in a proteins by very few amino acid substitutions. I propose to contrinue this research. I plan to collaborate with Dr. Donald Abraham at the University of Pittsburgh on X-ray studies of the binding sites of potential anti-sickling drugs in the haemoglobin molecule, with the aim of improving the design of such drugs on a rational basis and thus raising their potency. I plan to do further X-ray studies of other abnormal haemoglobins in order to learn more about the stereochemical basis of congenital diseases in general, and also about the allosteric mechanism and the intramolecular forces in haemoglobin.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL031461-02
Application #
3342588
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1983-12-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Medical Research Council Lab of Molec Biol
Department
Type
DUNS #
232560263
City
Cambridge
State
Country
United Kingdom
Zip Code
CB2 2QH
Boyle, W A; Muralidharan, S; Maher, G M et al. (1997) Vascular actions of 'caged' phenylephrine analogs depend on the structure and site of attachment of the 2-nitrobenzyl group. J Photochem Photobiol B 41:233-44
Stott, K; Blackburn, J M; Butler, P J et al. (1995) Incorporation of glutamine repeats makes protein oligomerize: implications for neurodegenerative diseases. Proc Natl Acad Sci U S A 92:6509-13
Whitaker, T L; Berry, M B; Ho, E L et al. (1995) The D-helix in myoglobin and in the beta subunit of hemoglobin is required for the retention of heme. Biochemistry 34:8221-6
Gilles-Gonzalez, M A; Gonzalez, G; Perutz, M F (1995) Kinase activity of oxygen sensor FixL depends on the spin state of its heme iron. Biochemistry 34:232-6
Perutz, M F (1995) Polar zippers: their role in human disease. Pharm Acta Helv 69:213-24
De Baere, I; Perutz, M F; Kiger, L et al. (1994) Formation of two hydrogen bonds from the globin to the heme-linked oxygen molecule in Ascaris hemoglobin. Proc Natl Acad Sci U S A 91:1594-7
Gilles-Gonzalez, M A; Gonzalez, G; Perutz, M F et al. (1994) Heme-based sensors, exemplified by the kinase FixL, are a new class of heme protein with distinctive ligand binding and autoxidation. Biochemistry 33:8067-73
Perutz, M (1994) Polar zippers: their role in human disease. Protein Sci 3:1629-37
Perutz, M F; Fermi, G; Poyart, C et al. (1993) A novel allosteric mechanism in haemoglobin. Structure of bovine deoxyhaemoglobin, absence of specific chloride-binding sites and origin of the chloride-linked Bohr effect in bovine and human haemoglobin. J Mol Biol 233:536-45
Gilles-Gonzalez, M A; Gonzalez, G (1993) Regulation of the kinase activity of heme protein FixL from the two-component system FixL/FixJ of Rhizobium meliloti. J Biol Chem 268:16293-7

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