Abstract

The major cause of vascular prosthesis failure is occlusion with thrombus related in part to the inherent thrombogenicity of artificial surfaces. Creation of an endothelial lining on the luminal surface of a vascular prosthesis would be beneficial towards the maintenance of long term patency of the graft. The major objective of the proposed research is to understand the interaction of human adult endothelial cells (EC) with substrate-treated vascular prosthetic surfaces as an initial step in understanding mechanisms of vascular graft endothelialization. These interactions will be evaluated with respect to the ability to maintain: - an adherent and morphologically confluent monolayer - a nonthrombogenic surface when EC are attached to prosthetic graft material and subjected to a physiological shear stress using human blood. The major aim of this research is to examine the following variables 1. EC origin (human capillaries vs. human large vessel EC) 2. EC isolation method (collagenase vs. mechanical scraping) 3. EC subcultivation (both method and number of subcultivations) 4. Vascular graft meterial (dacron vs. expanded PTFE) 5. Vascular graft substrate material (gelatin, collagen I/III, Collagen IV, blood, plasma and fibronectin) upon EC-prosthetic graft interactions as they relate to vascular graft endothelialization.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL033906-01A1
Application #
3346267
Study Section
Surgery and Bioengineering Study Section (SB)
Project Start
1986-07-01
Project End
1989-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Thomas Jefferson University
Department
Type
Schools of Medicine
DUNS #
061197161
City
Philadelphia
State
PA
Country
United States
Zip Code
19107

  Publications

Kelder, Steven H; Mitchell, Paul D; McKenzie, Thomas L et al. (2003) Long-term implementation of the CATCH physical education program. Health Educ Behav 30:463-75
Lytle, Leslie A; Ward, Jerri; Nader, Phillip R et al. (2003) Maintenance of a health promotion program in elementary schools: results from the CATCH-ON study key informant interviews. Health Educ Behav 30:503-18
Hoelscher, Deanna M; Mitchell, Paul; Dwyer, Johanna et al. (2003) How the CATCH eat smart program helps implement the USDA regulations in school cafeterias. Health Educ Behav 30:434-46
Johnson, Carolyn C; Li, Donglin; Galati, Todd et al. (2003) Maintenance of the classroom health education curricula: results from the CATCH-ON study. Health Educ Behav 30:476-88
Parcel, Guy S; Perry, Cheryl L; Kelder, Steven H et al. (2003) School climate and the institutionalization of the CATCH program. Health Educ Behav 30:489-502
Osganian, Stavroula K; Hoelscher, Deanna M; Zive, Michelle et al. (2003) Maintenance of effects of the eat smart school food service program: results from the CATCH-ON study. Health Educ Behav 30:418-33
Osganian, Stavroula K; Parcel, Guy S; Stone, Elaine J (2003) Institutionalization of a school health promotion program: background and rationale of the CATCH-ON study. Health Educ Behav 30:410-7
McKenzie, Thomas L; Li, Donglin; Derby, Carol A et al. (2003) Maintenance of effects of the CATCH physical education program: results from the CATCH-ON study. Health Educ Behav 30:447-62
Williams, S K; Schneider, T; Jarrell, B E (1990) Electron microscopy of endothelial cell-biopolymer interaction. Scanning Microsc 4:181-9
Freedman, D S; Srinivasan, S R; Harsha, D W et al. (1989) Relation of body fat patterning to lipid and lipoprotein concentrations in children and adolescents: the Bogalusa Heart Study. Am J Clin Nutr 50:930-9

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