Kidney contains renin and kallikrein bound to membranes. The enzymes are present in a plasma membrane enriched fraction obtained after differential centrifugation of the homogenized kidney cortex. The endoplasmic reticulum enriched fraction, however, also contains both enzymic activities. We will isolate the membranes the enzymes are bound to and establish the characteristics of the enzymes released from the membranes. We will study the process of activation and solubilization of renin and kallikrein and the various stimuli that lead to the release of the hypotensive and hypertensive enzymes.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
7R01HL036081-01
Application #
3350702
Study Section
Cardiovascular and Pulmonary Research B Study Section (CVB)
Project Start
1985-09-01
Project End
1988-08-31
Budget Start
1985-09-01
Budget End
1987-08-31
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
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