Recent studies suggest that lasers might be useful for the ablation of athero-sclerotic lesions, but the use of lasers for the endoscopic detection and quantitation of atherosclerosis has thus far received little attention. The proposed research is oriented towards the development of a fluorescence angioscopic technique capable of detecting and quantifying superficial and deep structural alterations of the arterial wall. Since the wall of atherosclerotic arteries may vary considerably in thickness due to the eccentricity of the arterial lumen, safe laser ablation in the region of arterial stenoses requires information about deep structure not provided by angioscopic visualization of the intima. The structural properties of isolated human artery segments with and without atherosclerosis will be determined by four independent morphometric techniques: 1) angioscopy, 2) casting of the lumen, 3) photography of the opened artery for the planimetry of intimal lesions, and 4) microscopic examination for the measurement of wall thickness, intimal thickness, and cross-sectional dimensions of atherosclerotic lesions. The intimal surface at arterial sites with defined structure will be irradiated with UV and visible lasers, and autofluorescence spectra will be recorded. Relative fluorescence intensities such as the ratio of fluorescence peaks will be related to the structural data. In addition, derived fluorescence parameters will be used to produce fluorescence maps of the arterial surface. Preliminiary experiments suggest that the fluorescence characteristics of arteries provides useful information about structural alterations of the arterial wall, and the proposed work should answer the question whether laser fluorescence imaging will be useful for the survey of arterial structure during angioplasty.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL036894-08
Application #
2218301
Study Section
Pathology A Study Section (PTHA)
Project Start
1986-07-01
Project End
1994-12-31
Budget Start
1994-01-01
Budget End
1994-12-31
Support Year
8
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Baylor College of Medicine
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
Bucay, M; Nguy, J; Barrios, R et al. (1998) Impaired adaptive vascular growth in hypercholesterolemic rabbit. Atherosclerosis 139:243-51
Chen, C H; Cartwright Jr, J; Li, Z et al. (1997) Inhibitory effects of hypercholesterolemia and ox-LDL on angiogenesis-like endothelial growth in rabbit aortic explants. Essential role of basic fibroblast growth factor. Arterioscler Thromb Vasc Biol 17:1303-12
Oraevsky, A A; Jacques, S L; Pettit, G H et al. (1993) XeCl laser ablation of atherosclerotic aorta: luminescence spectroscopy of ablation products. Lasers Surg Med 13:168-78
Oraevsky, A A; Jacques, S L; Pettit, G H et al. (1993) XeCl laser-induced fluorescence of atherosclerotic arteries. Spectral similarities between lipid-rich lesions and peroxidized lipoproteins. Circ Res 72:84-90
Pettit, G H; Sauerbrey, R; Tittel, F K et al. (1993) Atherosclerotic tissue analysis by time-resolved XeCl excimer laser reflectometry. Lasers Surg Med 13:279-83
Mangin Jr, E L; Kugiyama, K; Nguy, J H et al. (1993) Effects of lysolipids and oxidatively modified low density lipoprotein on endothelium-dependent relaxation of rabbit aorta. Circ Res 72:161-6
Oraevsky, A A; Jacques, S L; Pettit, G H et al. (1992) XeCl laser ablation of atherosclerotic aorta: optical properties and energy pathways. Lasers Surg Med 12:585-97
Yamamoto, H; Bossaller, C; Cartwright Jr, J et al. (1988) Videomicroscopic demonstration of defective cholinergic arteriolar vasodilation in atherosclerotic rabbit. J Clin Invest 81:1752-8
Sartori, M; Weilbaecher, D; Valderrama, G L et al. (1988) Laser-induced autofluorescence of human arteries. Circ Res 63:1053-9