Abstract

This work addresses the remodeling of pial blood vessels during the growth and differentiation of the brain and its internal vessels. Neural activity, adenosine, and rates of blood flow will be tested as interrelated factors that determine sprouting, enlargement, or regression of vessels. In the optic tectum of transparent Xenopus laevis tadpoles the diameters, velocities of flow,and shear rates will be followed in individual capillaries and venules with videomicroscopy and fluordscent tracers in vivo. Neural activity in the optic tectum will be altered during metamorphosis by intraocular TTX or enucleation. In the whisker barrel cortex of neonatal mice the regression of pial capillaries, pruning of arteriolar collaterals, and enlargement of specific arterioles and venules that supply internal capillaries will be documented. Selective manipulations to rows of whiskers will be used to test the effects of neural activity on vascular development. Adenosine agonists will be administered chronically intrathecally to test vascular growth. Adenosine antagonists will be administered chronically to hyperthernlic tadpoles and to selectively deafferented mice to test for inhibition of increased vascularization in response to higher levels of metabolism and neural activity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL041075-06
Application #
3358544
Study Section
Pathology A Study Section (PTHA)
Project Start
1988-07-01
Project End
1995-06-30
Budget Start
1993-07-01
Budget End
1994-06-30
Support Year
6
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Wei, L; Erinjeri, J P; Rovainen, C M et al. (2001) Collateral growth and angiogenesis around cortical stroke. Stroke 32:2179-84
Moskalenko YuE; Rovainen, C; Woolsey, T A et al. (1996) Comparison of measurements of local brain blood flow by hydrogen clearance with the inhalation of hydrogen and its electrochemical generation in brain tissue. Neurosci Behav Physiol 26:245-50
Moskalenko, Y E; Dowling, J L; Liu, D et al. (1996) LCBF changes in rat somatosensory cortex during whisker stimulation monitored by dynamic H2 clearance. Int J Psychophysiol 21:45-59
Tang, Y Y; Rovainen, C M (1996) Cardiac output in Xenopus laevis tadpoles during development and in response to an adenosine agonist. Am J Physiol 270:R997-1004
Rovainen, C M (1996) Feeding and breathing in lampreys. Brain Behav Evol 48:297-305
Wei, L; Rovainen, C M; Woolsey, T A (1995) Ministrokes in rat barrel cortex. Stroke 26:1459-62
Moskalenko, Iu E; Rovainen, C M; Woolsey, T A et al. (1994) [A comparison of the measurements of local cerebral blood flow by hydrogen clearance with hydrogen inhalation and by its electrochemical generation in brain tissue] Fiziol Zh Im I M Sechenova 80:119-26
Jen, S C; Rovainen, C M (1994) An adenosine agonist increases blood flow and density of capillary branches in the optic tectum of Xenopus laevis tadpoles. Microcirculation 1:59-66
Moskalenko, Iu E; Rovainen, C M; Woolsey, T A et al. (1994) [Combinations of methods for the monitoring of the cerebral microcirculation] Fiziol Zh Im I M Sechenova 80:144-53
Cox, S B; Woolsey, T A; Rovainen, C M (1993) Localized dynamic changes in cortical blood flow with whisker stimulation corresponds to matched vascular and neuronal architecture of rat barrels. J Cereb Blood Flow Metab 13:899-913

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