Several discrete lines of study on the structure and biosynthesis of airway mucin will be followed: 1. Cloning of the cDNA for the polypeptide core of human and canine tracheal mucin glycoproteins. Knowledge of the molecular weight of the deglycosylated materials coupled with the availability of antibodies directed against the proteins makes this feasible. Work will be initiated using an appropriate cDNA library and following identification, cloning and expansion, the cDNA sequence will be determined. The cDNAs thus obtained will be utilized for isolation of genomic clones which also will be sequenced. Clone identity will be confirmed by amino acid sequence analysis of selected peptide fragments. The latter data will also be continued with results of monoclonal antibody studies to prepare synthetic nucleotide probes from domains unique to the tracheal mucin glycoprotein. 2. Completion of the description of the organization of the macrostructure of the tracheobronchial mucins.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL042332-04
Application #
3360464
Study Section
Special Emphasis Panel (SRC)
Project Start
1988-07-01
Project End
1993-04-30
Budget Start
1991-05-01
Budget End
1992-04-30
Support Year
4
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Georgetown University
Department
Type
Schools of Dentistry
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057
Verma, M; Davidson, E A (1994) Canine U2 snRNA gene: nucleotide sequence, characterization and implications in RNA processing and cancer biology. Cancer Biochem Biophys 14:123-31
Verma, M; Davidson, E A (1993) Molecular cloning and sequencing of a canine tracheobronchial mucin cDNA containing a cysteine-rich domain. Proc Natl Acad Sci U S A 90:7144-8
Bhargava, A K; Woitach, J T; Davidson, E A et al. (1990) Cloning and cDNA sequence of a bovine submaxillary gland mucin-like protein containing two distinct domains. Proc Natl Acad Sci U S A 87:6798-802