Abstract

.) It is hypothesized that the cellular and temporal pattern of expression of the GRP receptor is critical in determining the action of the peptide. This will be studied through a program focused on the characterization of the gastrin releasing peptide receptor. Furthermore, antibodies directed against the GRP receptor will be produced and used as highly specific antagonists.
Specific Aims i nclude the following: 1. Development of immunologic and molecular reagents with which to study GRP receptor. 2. Analysis of GRP receptor cDNA sequence and deduction of GRP receptor structure and function. 3. Descriptive studies of GRP receptor expression. 4. Interference of GRP action in lung development using receptor-directed agents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL045745-04
Application #
2222464
Study Section
Pathology A Study Section (PTHA)
Project Start
1991-01-01
Project End
1995-12-31
Budget Start
1994-01-01
Budget End
1994-12-31
Support Year
4
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Cohen, A J; King Jr, T E; Gilman, L B et al. (1998) High expression of neutral endopeptidase in idiopathic diffuse hyperplasia of pulmonary neuroendocrine cells. Am J Respir Crit Care Med 158:1593-9
Miller, Y E; Franklin, W A (1997) Molecular events in lung carcinogenesis. Hematol Oncol Clin North Am 11:215-34
Girod, C E; Shin, D H; Hershenson, M B et al. (1996) p172: An alveolar type II and Clara cell specific protein with late developmental expression and upregulation by hyperoxic lung injury. Am J Respir Cell Mol Biol 14:538-47
Cohen, A J; Bunn, P A; Franklin, W et al. (1996) Neutral endopeptidase: variable expression in human lung, inactivation in lung cancer, and modulation of peptide-induced calcium flux. Cancer Res 56:831-9
Shenkar, R; Cohen, A J; Vestweber, D et al. (1995) Hemorrhage and resuscitation alter the expression of ICAM-1 and P-selectin in mice. J Inflamm 45:248-59
Geraci, M W; Miller, Y E; Escobedo-Morse, A et al. (1994) Novel bombesin-like peptide binding proteins from lung. Am J Respir Cell Mol Biol 10:331-8
Cook, R M; Burke, B J; Buchhagen, D L et al. (1993) Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer. J Biol Chem 268:17010-7
Aguayo, S M; King Jr, T E; Kane, M A et al. (1992) Urinary levels of bombesin-like peptides in asymptomatic cigarette smokers: a potential risk marker for smoking-related diseases. Cancer Res 52:2727s-2731s
Aguayo, S M; Miller, Y E; Waldron Jr, J A et al. (1992) Brief report: idiopathic diffuse hyperplasia of pulmonary neuroendocrine cells and airways disease. N Engl J Med 327:1285-8