Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
2R01HL047415-05
Application #
2223643
Study Section
Biophysical Chemistry Study Section (BBCB)
Project Start
1992-08-01
Project End
2000-07-31
Budget Start
1996-08-01
Budget End
1997-07-31
Support Year
5
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
Krykbaev, Rustem A; Tsantili, Panayota; Jeffrey, Philip D et al. (2002) Modifying specificity of antidigoxin antibodies using insertional mutagenesis. Protein Sci 11:2899-908
Parhami-Seren, Behnaz; Viswanathan, Malini; Margolies, Michael N (2002) Selection of high affinity p-azophenyarsonate Fabs from heavy-chain CDR2 insertion libraries. J Immunol Methods 259:43-53
Parhami-Seren, Behnaz; Haberly, Richard; Margolies, Michael N et al. (2002) Ouabain-binding protein(s) from human plasma. Hypertension 40:220-8
Short, Mary K; Krykbaev, Rustem A; Jeffrey, Philip D et al. (2002) Complementary combining site contact residue mutations of the anti-digoxin Fab 26-10 permit high affinity wild-type binding. J Biol Chem 277:16365-70
Short, M K; Jeffrey, P D; Demirjian, A et al. (2001) A single H:CDR3 residue in the anti-digoxin antibody 26-10 modulates specificity for C16-substituted digoxin analogs. Protein Eng 14:287-96
Krykbaev, R A; Liu, W R; Jeffrey, P D et al. (2001) Phage display-selected sequences of the heavy-chain CDR3 loop of the anti-digoxin antibody 26-10 define a high affinity binding site for position 16-substituted analogs of digoxin. J Biol Chem 276:8149-58
Parhami-Seren, B; Viswanathan, M; Strong, R K et al. (2001) Structural analysis of mutants of high-affinity and low-affinity p-azophenylarsonate-specific antibodies generated by alanine scanning of heavy chain complementarity-determining region 2. J Immunol 167:5129-35
Kim, S H; Titlow, C C; Margolies, M N (2000) An approach for preventing recombination-deletion of the 40-50 anti-digoxin antibody V(H) gene from the phage display vector pComb3. Gene 241:19-25
Ball Jr, W J; Wang, Z; Malik, B et al. (2000) Selection of peptidic mimics of digoxin from phage-displayed peptide libraries by anti-digoxin antibodies. J Mol Biol 301:101-15
Roy, P; Roth, C M; Margolies, M N et al. (2000) Aromatic residues mediate the pressure-induced association of digoxigenin and antibody 26-10. Biophys Chem 83:171-7

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