Abstract

These investigators propose to design and synthesize a series of bifunctional organic reagents which contain negatively charged functional groups and range of specific molecular tether lengths. These bifunctional organic reagents are for chemically modifying cell-free human hemoglobins for use as oxygen carrying blood substitutes in emergency transfusions. These artificial oxygen carriers would be free of HIV and other transfusion-transmitted infectious agents. The investigators will verify molecular structures configuration, conformation and tether length of the newly synthesized reagents and then explore the reagents' potential to form covalent bonds with natural amino acids lysine cysteine valine in aqueous media. The modified hemoglobins will be purified and their physical composition and functional properties will be investigated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL048632-01A2
Application #
2224702
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
1994-09-30
Project End
1996-08-31
Budget Start
1994-09-30
Budget End
1995-08-31
Support Year
1
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Chemistry
Type
Schools of Medicine
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201