Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL052096-02
Application #
2229259
Study Section
Biophysical Chemistry Study Section (BBCB)
Project Start
1995-07-01
Project End
1998-06-30
Budget Start
1996-07-01
Budget End
1997-06-30
Support Year
2
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Leonidas, Demetres D; Shapiro, Robert; Subbarao, Gowtham V et al. (2002) Crystallographic studies on the role of the C-terminal segment of human angiogenin in defining enzymatic potency. Biochemistry 41:2552-62
Jardine, A M; Leonidas, D D; Jenkins, J L et al. (2001) Cleavage of 3',5'-pyrophosphate-linked dinucleotides by ribonuclease A and angiogenin. Biochemistry 40:10262-72
Leonidas, D D; Chavali, G B; Jardine, A M et al. (2001) Binding of phosphate and pyrophosphate ions at the active site of human angiogenin as revealed by X-ray crystallography. Protein Sci 10:1669-76
Russo, A; Acharya, K R; Shapiro, R (2001) Small molecule inhibitors of RNase A and related enzymes. Methods Enzymol 341:629-48
Shapiro, R; Ruiz-Gutierrez, M; Chen, C Z (2000) Analysis of the interactions of human ribonuclease inhibitor with angiogenin and ribonuclease A by mutagenesis: importance of inhibitor residues inside versus outside the C-terminal ""hot spot"". J Mol Biol 302:497-519
Leonidas, D D; Shapiro, R; Allen, S C et al. (1999) Refined crystal structures of native human angiogenin and two active site variants: implications for the unique functional properties of an enzyme involved in neovascularisation during tumour growth. J Mol Biol 285:1209-33
Chen, C Z; Shapiro, R (1999) Superadditive and subadditive effects of ""hot spot"" mutations within the interfaces of placental ribonuclease inhibitor with angiogenin and ribonuclease A. Biochemistry 38:9273-85
Shapiro, R (1998) Structural features that determine the enzymatic potency and specificity of human angiogenin: threonine-80 and residues 58-70 and 116-123. Biochemistry 37:6847-56
Leonidas, D D; Shapiro, R; Irons, L I et al. (1997) Crystal structures of ribonuclease A complexes with 5'-diphosphoadenosine 3'-phosphate and 5'-diphosphoadenosine 2'-phosphate at 1.7 A resolution. Biochemistry 36:5578-88
Soncin, F; Strydom, D J; Shapiro, R (1997) Interaction of heparin with human angiogenin. J Biol Chem 272:9818-24

Showing the most recent 10 out of 14 publications