(Verbatim from the application): The goal of these studies is to use in vivo panning methods with phage display libraries to identify homing peptide for the heart neovasculature, the coronary endothelium and the endocardium. We wish to use these peptides, especially the prototype RGD neovascular homing peptide, as ligands to target retrovirus and HIV lentivirus vectors to those tissues. We will also examine a method for selecting more efficient RGD-targeted vectors from a combinatorial retrovirus library. Finally, we propose to characterize the biodistribution of these targeted vectors by sensitive fluorescence methods and correlate vector attachment with sites of endothelial-specific gene expression.
| Guibinga, Ghiabe H; Friedmann, Theodore (2005) Baculovirus GP64-pseudotyped HIV-based lentivirus vectors are stabilized against complement inactivation by codisplay of decay accelerating factor (DAF) or of a GP64-DAF fusion protein. Mol Ther 11:645-51 |
| Guibinga, Ghiabe H; Hall, Frederick L; Gordon, Erlinda M et al. (2004) Ligand-modified vesicular stomatitis virus glycoprotein displays a temperature-sensitive intracellular trafficking and virus assembly phenotype. Mol Ther 9:76-84 |
| Guibinga, Ghiabe H; Miyanohara, Atsushi; Esko, Jeffrey D et al. (2002) Cell surface heparan sulfate is a receptor for attachment of envelope protein-free retrovirus-like particles and VSV-G pseudotyped MLV-derived retrovirus vectors to target cells. Mol Ther 5:538-46 |
| Okimoto, T; Friedmann, T; Miyanohara, A (2001) VSV-G envelope glycoprotein forms complexes with plasmid DNA and MLV retrovirus-like particles in cell-free conditions and enhances DNA transfection. Mol Ther 4:232-8 |
