Abstract

This Facility will provide comprehensive support for microarray-based studies related to NHLBI funded research programs at the University of California San Francisco (UCSF). UCSF is a major site for NHLBI-funded research. Strong institutional support from UCSF and the affiliated Gladstone Institutes enabled us to establish a microarray facility and gain experience with GeneChip and spotted arrays. Recent technology development efforts in the Facility have focused on production and validation of human and mouse spotted oligonucleotide arrays and collaborative development of a web-based open source microarray database system. This grant, coupled with ongoing institutional support, will enable us to expand the Facility to allow integration of GeneChip and spotted oligonucleotide array technology into many NHLBI-supported projects. This application includes eleven proposals for microarray studies involving projects directed by 14 UCSF investigators and funded by a total of 17 different NHLBI grants. We also describe plans for supporting new NHLBI-funded projects. Each project will collaborate closely with the Facility to surmount the challenges posed by microarray technology and to use the technology to generate and test important and novel hypotheses. The Facility will provide technical training and consultation and cost-effective access to arrays and related equipment. Experts in bioinformatics and biostatistics will promote the use of state of the art approaches to data analysis and dissemination. The Facility will also take an active role in implementing new methods that will be useful for all participating projects. Designation of our Facility as a Shared Microarray Facility will have a major impact on a large and productive group of NHLBI-supported researchers at UCSF.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL072301-04
Application #
6930480
Study Section
Special Emphasis Panel (ZHL1-CSR-D (S1))
Program Officer
Ye, Jane
Project Start
2002-09-30
Project End
2007-07-31
Budget Start
2005-08-01
Budget End
2007-07-31
Support Year
4
Fiscal Year
2005
Total Cost
$665,540
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Winn, Virginia D; Gormley, Matthew; Fisher, Susan J (2011) The Impact of Preeclampsia on Gene Expression at the Maternal-Fetal Interface. Pregnancy Hypertens 1:100-8
Winn, Virginia D; Gormley, Matthew; Paquet, Agnes C et al. (2009) Severe preeclampsia-related changes in gene expression at the maternal-fetal interface include sialic acid-binding immunoglobulin-like lectin-6 and pappalysin-2. Endocrinology 150:452-62
Lewis, Christina C; Yang, Jean Yee Hwa; Huang, Xiaozhu et al. (2008) Disease-specific gene expression profiling in multiple models of lung disease. Am J Respir Crit Care Med 177:376-87
Wagner, Amy J; Stumbaugh, Amber; Tigue, Zachary et al. (2008) Genetic analysis of congenital cystic adenomatoid malformation reveals a novel pulmonary gene: fatty acid binding protein-7 (brain type). Pediatr Res 64:11-6
Woodruff, Prescott G; Boushey, Homer A; Dolganov, Gregory M et al. (2007) Genome-wide profiling identifies epithelial cell genes associated with asthma and with treatment response to corticosteroids. Proc Natl Acad Sci U S A 104:15858-63
Araya, Jun; Cambier, Stephanie; Markovics, Jennifer A et al. (2007) Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients. J Clin Invest 117:3551-62
Zhen, Guohua; Park, Sung Woo; Nguyenvu, Louis T et al. (2007) IL-13 and epidermal growth factor receptor have critical but distinct roles in epithelial cell mucin production. Am J Respir Cell Mol Biol 36:244-53
Winn, Virginia D; Haimov-Kochman, Ronit; Paquet, Agnes C et al. (2007) Gene expression profiling of the human maternal-fetal interface reveals dramatic changes between midgestation and term. Endocrinology 148:1059-79
Mallen-St Clair, Jon; Shi, Guo-Ping; Sutherland, Rachel E et al. (2006) Cathepsins L and S are not required for activation of dipeptidyl peptidase I (cathepsin C) in mice. Biol Chem 387:1143-6
Woodruff, Prescott G; Koth, Laura L; Yang, Yee Hwa et al. (2005) A distinctive alveolar macrophage activation state induced by cigarette smoking. Am J Respir Crit Care Med 172:1383-92

Showing the most recent 10 out of 18 publications