Both in situ synthesized oligonucleotide and cDNA spotted microarrays provide important tools, which need to be made generally available to the NHLBI community at high and consistent quality and at a reasonable price through the shared resource. The proposed NHLBI Shared Resource within the UCLA DNA Microarray Facility will be a broadly functioning center to provide an array of microarray reagents and services. The current UCLA Microarray core provides a full service from microarray performance to data management and delivery. With broad campus support the UCLA DNA Microarray Facility has been functioning to provide microarray based gene expression analysis using spotted cDNA and in situ synthesized oligo arrays. In this application we propose an extension of these services: 1) To provide assistance in experimental design. We will offer a series of educational programs at UCLA with the overall goal of creating a knowledgeable microarray user community. 2) To provide microarray services to meet demands of NHLBI investigator base. The current facility will be expanded to meet the needs for increased capacity. 3) To provide data management and statistical analysis of microarray data. As large scale data handling and processing can be a daunting task for biologists, we propose to provide dedicated expert data managing and statistical assistance.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL072367-02
Application #
6667247
Study Section
Special Emphasis Panel (ZHL1-CSR-D (S1))
Program Officer
Ye, Jane
Project Start
2002-09-30
Project End
2006-07-31
Budget Start
2003-08-01
Budget End
2004-07-31
Support Year
2
Fiscal Year
2003
Total Cost
$735,780
Indirect Cost
Name
University of California Los Angeles
Department
Pediatrics
Type
Schools of Medicine
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Pellegrini, Matteo; Cheng, Jerry C; Voutila, Jon et al. (2008) Expression profile of CREB knockdown in myeloid leukemia cells. BMC Cancer 8:264
Funari, Vincent A; Day, Allen; Krakow, Deborah et al. (2007) Cartilage-selective genes identified in genome-scale analysis of non-cartilage and cartilage gene expression. BMC Genomics 8:165
Day, Allen; Carlson, Marc R J; Dong, Jun et al. (2007) Celsius: a community resource for Affymetrix microarray data. Genome Biol 8:R112
Lee, Hans; Zhang, Yanqiao; Lee, Florence Y et al. (2006) FXR regulates organic solute transporters alpha and beta in the adrenal gland, kidney, and intestine. J Lipid Res 47:201-14
Gargalovic, Peter S; Imura, Minori; Zhang, Bin et al. (2006) Identification of inflammatory gene modules based on variations of human endothelial cell responses to oxidized lipids. Proc Natl Acad Sci U S A 103:12741-6
Mitchell, Scherise; Ota, Asuka; Foster, William et al. (2006) Distinct gene expression profiles in adult mouse heart following targeted MAP kinase activation. Physiol Genomics 25:50-9