Abstract

Heart disease remains the leading cause of morbidity and mortality in the United States, responsible for over 1,400,000 deaths in 2001. Limitations in technology have hampered our ability to ameliorate the impact of an important group of cardiac disorders, congenital heart disease. The multi-disciplinary project described in this five-year proposal will develop and validate new investigative and diagnostic technologies based on the emerging imaging technique, optical coherence tomography (OCT). OCT is capable of simultaneous imaging of structure and function in small biological samples with micrometer-scale resolution in vivo and in real time, and is potentially capable of measuring electromechanical events. A multi-disciplinary approach is necessary to target the development of these new tools for the purpose of improving our understanding of the mechanisms of congenital heart disease.
Specific Aim 1 : Develop and demonstrate OCT imaging technologies for developmental cardiology research applications and disseminate to collaborators for validation and baseline studies.
Specific Aim 2 : Develop and demonstrate image analysis and visualization methods and disseminate to collaborators for validation and baseline studies.
Specific Aim 3 : Validate and apply OCT technologies to investigations of normal and abnormal cardiac morphogenesis in the chick embryo. Longitudinal analysis.
Specific Aim 4 : Validate and apply OCT technologies to screen and analyze cardiac defects in genetically altered mouse lines. Analysis of conotruncal and ventricular wall abnormalities. The goal of this project is to develop and validate a novel set of tools that will combine structural and physiologic information at heretofore unachievable resolution scales for better insight into the mechanisms of normal and abnormal cardiac morphogenesis. The utility of these tools to enable experiments that were previously not possible will be established by baseline studies of chick and mouse models of normal and abnormal cardiac morphogenesis. ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL083048-03
Application #
7472295
Study Section
Biomedical Imaging Technology Study Section (BMIT)
Program Officer
Buxton, Denis B
Project Start
2006-08-01
Project End
2010-07-31
Budget Start
2008-08-01
Budget End
2009-07-31
Support Year
3
Fiscal Year
2008
Total Cost
$371,408
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Elahi, Sahar; Gu, Shi; Thrane, Lars et al. (2018) Complex regression Doppler optical coherence tomography. J Biomed Opt 23:1-8
Thrane, Lars; Gu, Shi; Blackburn, Brecken J et al. (2017) Complex decorrelation averaging in optical coherence tomography: a way to reduce the effect of multiple scattering and improve image contrast in a dynamic scattering medium. Opt Lett 42:2738-2741
Karunamuni, Ganga; Sheehan, Megan M; Doughman, Yong Qiu et al. (2017) Supplementation with the Methyl Donor Betaine Prevents Congenital Defects Induced by Prenatal Alcohol Exposure. Alcohol Clin Exp Res 41:1917-1927
Ford, Stephanie M; McPheeters, Matthew T; Wang, Yves T et al. (2017) Increased regurgitant flow causes endocardial cushion defects in an avian embryonic model of congenital heart disease. Congenit Heart Dis 12:322-331
Watanabe, Michiko; Rollins, Andrew M; Polo-Parada, Luis et al. (2016) Probing the Electrophysiology of the Developing Heart. J Cardiovasc Dev Dis 3:
Ma, Pei; Chan, Dennis C; Gu, Shi et al. (2016) Volumetric optical mapping in early embryonic hearts using light-sheet microscopy. Biomed Opt Express 7:5120-5128
Blech-Hermoni, Yotam; Sullivan, Connor B; Jenkins, Michael W et al. (2016) CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart. Dev Dyn 245:854-73
Wang, Yves T; Rollins, Andrew M; Jenkins, Michael W (2016) Infrared inhibition of embryonic hearts. J Biomed Opt 21:60505
Ma, Pei; Gu, Shi; Karunamuni, Ganga H et al. (2016) Cardiac neural crest ablation results in early endocardial cushion and hemodynamic flow abnormalities. Am J Physiol Heart Circ Physiol 311:H1150-H1159
Karunamuni, Ganga; Gu, Shi; Doughman, Yong Qiu et al. (2015) Using optical coherence tomography to rapidly phenotype and quantify congenital heart defects associated with prenatal alcohol exposure. Dev Dyn 244:607-18

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