Abstract

Recently developed electroanalytical techniques are to be used to provide a new understanding of the static and dynamic functioning of catecholamines and related substances in small animal brains. Microelectrodes, improved in selectivity and quantitative response, will be used to upgrade the already reliable in vivo electrochemistry methodology. This technique measure changes in concentration of catecholamines and related substances in brain extracellular fluid. Using an inexpensive microprocessor to control simultaneous multiple site electrochemical recording, we will begin mapping functional chemical changes in brain areas following sensory and environmental stimulation of unanesthetized, unrestrained rats. High sensitivity liquid chromatography with electrochemical detection in conjunction with punching techniques has uncovered unusual concentration gradients and strong chemical lateralization (left-right hemispheric differences) of catecholamines in discrete rat brain areas. We will provide detailed quantitative mapping of these distribution patterns for catecholamines and ascorbate in rat cortex, thalamus and other inner core structures. Neurotransmitter concentration profiles and, especially, hemispheric differences are practically uncharted in small animal brains. The biogenic amine distribution patterns may also help explain dynamic chemical responses seen with the in vivo electrodes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS008740-19
Application #
3393805
Study Section
(SSS)
Project Start
1976-09-20
Project End
1985-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
19
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Kansas Lawrence
Department
Type
Schools of Arts and Sciences
DUNS #
072933393
City
Lawrence
State
KS
Country
United States
Zip Code
66045

  Publications

Mitchell, K; Oke, A F; Adams, R N (1994) In vivo dynamics of norepinephrine release-reuptake in multiple terminal field regions of rat brain. J Neurochem 63:917-26
Mitchell, K; Adams, R N (1993) Comparison of the effects of voltage-sensitive calcium channel antagonism on the electrically stimulated release of dopamine and norepinephrine in vivo. Brain Res 604:349-53
Capella, P; Ghasemzadeh, M B; Adams, R N et al. (1993) Real-time monitoring of electrically stimulated norepinephrine release in rat thalamus: II. Modeling of release and reuptake characteristics of stimulated norepinephrine overflow. J Neurochem 60:449-53
Ghasemzadeh, M B; Capella, P; Mitchell, K et al. (1993) Real-time monitoring of electrically stimulated norepinephrine release in rat thalamus: I. Resolution of transmitter and metabolite signal components. J Neurochem 60:442-8
Cammack, J; Ghasemzadeh, B; Adams, R N (1992) Electrochemical monitoring of brain ascorbic acid changes associated with hypoxia, spreading depression, and seizure activity. Neurochem Res 17:23-7
Oke, A F; Putz, C; Adams, R N et al. (1992) Neuroleptic treatment is an unlikely cause of elevated dopamine in thalamus of schizophrenic subjects. Psychiatry Res 45:203-8
Renner, K J; Pazos, L; Adams, R N (1992) In vivo voltammetric evidence for the detection of norepinephrine release in the thalamus of freely moving rats. Brain Res 577:49-56
Cammack, J; Oke, A; Adams, R N (1991) Simultaneous high-performance liquid chromatographic determination of ascorbic acid and dehydroascorbic acid in biological samples. J Chromatogr 565:529-32
Cammack, J; Ghasemzadeh, B; Adams, R N (1991) The pharmacological profile of glutamate-evoked ascorbic acid efflux measured by in vivo electrochemistry. Brain Res 565:17-22
Rice, M E; Cammack, J (1991) Anoxia-resistant turtle brain maintains ascorbic acid content in vitro. Neurosci Lett 132:141-5

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