The general aim of this investigation is to determine some of the factors that influence axonal growth during regeneration. Particular emphasis is given to studies of goldfish optic axons, which show exceptionally vigorous regeneration, with the hope that these studies may provide clues to procedures for enhancing axonal outgrowth and thus promoting recovery from damage to the central system in mammals. Some of the topics under investigation are the mechanisms of axonal transport in normal and regenerating cells, changes in axonal transport of calcium during regeneration, interactions between growing axon tip and the nerve cell body and the action of Nerve Growth Factor on regenerating cells. Also, the reaction of mammalian retinal ganglion cells to axotomy will be compared to the reaction of the corresponding cells in goldfish. The methods to be used will include radioactive labeling, light microscopy, electron microscopy, and behavioral testing.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS009015-18
Application #
3393875
Study Section
Neurology B Subcommittee 1 (NEUB)
Project Start
1977-09-01
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
18
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Weill Medical College of Cornell University
Department
Type
Schools of Medicine
DUNS #
201373169
City
New York
State
NY
Country
United States
Zip Code
10065
Perry, G W; Burmeister, D W; Grafstein, B (1990) Effect of target removal on goldfish optic nerve regeneration: analysis of fast axonally transported proteins. J Neurosci 10:3439-48
Barron, K D; McGuinness, C M; Misantone, L J et al. (1985) RNA content of normal and axotomized retinal ganglion cells of rat and goldfish. J Comp Neurol 236:265-73