Abstract

Antibodies will be raised that are specific for a potent activator of the acetylcholine receptor (AChR), trans-3,3'-BisQ. The specificity of these antibodies will be determined with respect to other compounds that bind to AChR in order to determine whether homology of binding exists in AChR and the specific antibody. If so, the antisera will be used to screen for other cholinergic compounds. An attempt will also be made to prepare homogeneous globulin (i.e. antibody) protein specific for trans-3, 3'-BisQ to further characterize its binding properties. Finally, anti-idiotypic antibodies will be prepared and its specificity for AChR determined. All of the objectives are related to gaining an understanding of the function of AChR in health and disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS015581-11
Application #
3396343
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1979-09-24
Project End
1992-03-31
Budget Start
1990-09-01
Budget End
1992-03-31
Support Year
11
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Type
Schools of Medicine
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027

  Publications

Salmon, J E; Brogle, N; Brownlie, C et al. (1993) Human mononuclear phagocytes express adenosine A1 receptors. A novel mechanism for differential regulation of Fc gamma receptor function. J Immunol 151:2775-85
Cacalano, N A; Aggarwal, R; Quesniaux, V F et al. (1992) Novel monoclonal antibodies to cyclosporine A: characterization and epitope mapping with cyclosporine analogs and cyclophilin. Mol Immunol 29:107-18
Erlanger, B F (1992) Do we know the site of action of cyclosporin? Immunol Today 13:487-90
Cacalano, N A; Chen, B X; Cleveland, W L et al. (1992) Evidence for a functional receptor for cyclosporin A on the surface of lymphocytes. Proc Natl Acad Sci U S A 89:4353-7
Taub, R; Hsu, J C; Garsky, V M et al. (1992) Peptide sequences from the hypervariable regions of two monoclonal anti-idiotypic antibodies against the thyrotropin (TSH) receptor are similar to TSH and inhibit TSH-increased cAMP production in FRTL-5 thyroid cells. J Biol Chem 267:5977-84
el-Etr, M; Lombes, M; Baulieu, E E et al. (1992) A monoclonal anti-idiotypic 'internal image' antibody that recognizes the A1 adenosine receptor potentiates the alpha 1-adrenergic activation of phospholipase C in primary cultures of mouse striatal astrocytes. Neurosci Lett 145:15-8
Chen, B X; Kubo, K; Ide, H et al. (1992) Properties of a monoclonal antibody for the detection of abasic sites, a common DNA lesion. Mutat Res 273:253-61
Cacalano, N A; Cleveland, W L; Erlanger, B F (1991) Characterization of a monoclonal anti-idiotypic antibody that mimics cyclosporine A in a single binding system. J Immunol 147:3012-7
Lombes, M; Farman, N; Oblin, M E et al. (1990) Immunohistochemical localization of renal mineralocorticoid receptor by using an anti-idiotypic antibody that is an internal image of aldosterone. Proc Natl Acad Sci U S A 87:1086-8
Lombes, M; Desfosses, B; Cittanova, N et al. (1989) A syngeneic monoclonal anti-idiotypic antibody with internal image properties restricted to a single aldosterone-binding system. J Immunol 143:4078-83

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