The purpose of this proposal is to continue our investigation of the intrinsic neural organization of three nuclei in the medial hypothalamus. Specifically, we will focus our attention on the suprachiasmatic, paraventricular, and arcuate nuclei. We plan to use a combination of Golgi impregnations and immunocytochemistry to study the dendritic arbors, axonal ramifications, and synaptic communication between neurons resident in this part of the brain. Our first step will be to examine with computer-assistance the frequency and orientation of immunostained axons labeled with antibodies directed against peptides endodgenous to this part of the hypothalamus. Secondly, we will compare Golgi impregnated and immunocytochemically stained dendritic trees in different parts of each nucleus. Third, we will use double immunostaining at the ultrastructural level to determine what cells communicate with which other cells. Finally, we will use a combination of retrograde dye transport (fast blue) with immunocytochemistry to determine the location and specific peptide synthesized of cells which project to the neurohypophysis and median eminence. These studies are critical for our understanding the morphological substrates of hypothalamic function.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS016296-06
Application #
3396794
Study Section
Neurology B Subcommittee 1 (NEUB)
Project Start
1980-03-01
Project End
1987-03-31
Budget Start
1985-04-01
Budget End
1987-03-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Yale University
Department
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
Obrietan, K; van den Pol, A N (1996) Neuropeptide Y depresses GABA-mediated calcium transients in developing suprachiasmatic nucleus neurons: a novel form of calcium long-term depression. J Neurosci 16:3521-33
van den Pol, A N; Strecker, G J; Dudek, F E (1996) Excitatory and inhibitory amino acids and synaptic transmission in the suprachiasmatic nucleus. Prog Brain Res 111:41-56
Romano, C; van den Pol, A N; O'Malley, K L (1996) Enhanced early developmental expression of the metabotropic glutamate receptor mGluR5 in rat brain: protein, mRNA splice variants, and regional distribution. J Comp Neurol 367:403-12
Chen, G; Trombley, P Q; van den Pol, A N (1995) GABA receptors precede glutamate receptors in hypothalamic development;differential regulation by astrocytes. J Neurophysiol 74:1473-84
Romano, C; Sesma, M A; McDonald, C T et al. (1995) Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain. J Comp Neurol 355:455-69
van den Pol, A N; Romano, C; Ghosh, P (1995) Metabotropic glutamate receptor mGluR5 subcellular distribution and developmental expression in hypothalamus. J Comp Neurol 362:134-50
Obrietan, K; Van den Pol, A N (1995) Calcium hyperexcitability in neurons cultured with glutamate receptor blockade. J Neurophysiol 73:1524-36
Obrietan, K; Belousov, A B; Heller, H C et al. (1995) Adenosine pre- and postsynaptic modulation of glutamate-dependent calcium activity in hypothalamic neurons. J Neurophysiol 74:2150-62
van den Pol, A N (1995) Presynaptic metabotropic glutamate receptors in adult and developing neurons: autoexcitation in the olfactory bulb. J Comp Neurol 359:253-71
van den Pol, A N; Hermans-Borgmeyer, I; Hofer, M et al. (1994) Ionotropic glutamate-receptor gene expression in hypothalamus: localization of AMPA, kainate, and NMDA receptor RNA with in situ hybridization. J Comp Neurol 343:428-44

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