A number of afferent and efferent projections of the cerebellar cortex have been shown to terminate as a series of parasagittally oriented zones. The concept of a """"""""cerebellar module"""""""" or """"""""microzone"""""""" has been proposed as being the basic functional unit of the cerebellum which modulates a certain aspect of motor behavior. The experiments in this proposal are designed to test hypotheses relating to the concept of a """"""""cerebellar module"""""""" and to discern the organizational principles of brainstem afferents to the posterior cerebellar cortex in the rat and mouse. These experiments are as follows: 1. Inject individual brainstem nuclei with tritiated amino acids to determine the organization of their inputs to the cerebellar cortex. 2. Inject wheat germ agglutinin-horseradish peroxidase (WGA-HRP) conjugate into different mediolateral positions in the same and different cerebellar lobules to map the location, of brainstem cells which project to these specific regions. 3. Inject to retrograde tracers into two predetermined parasagital zones to determine whether individual neurons project oxon collaterals to more than one zone. 4. Inject WGA-HRP in the cerebellar cortex and tritiated amino acids in the brainstem of the same animal to corroborate the results of the studies listed above. 5. Inject two retrograde tracers, one in cerebellar cortex and one in the deep cerebellar nuclei to determine if olivary cells project collaterals onto deep nuclear cells which receive afferents from the Purkinje cells innervated by the same group of olivary cells. The careful execution of the experiments should provide interesting and exciting information concerning the anotomical organization of afferent input to the posterior cerebellar cortex. It should also provide some important data upon which we can evaluate the concept of the """"""""cerebellar module"""""""" as the basic functional unit of the cerebellum and some information concerning the nature of that function. Hopefully this information will lead to a clearer understanding of the cerebllum's role in modulating motor activity in the normal animal and in animals with cerebellar or other motor diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS016531-03
Application #
3396952
Study Section
Neurology A Study Section (NEUA)
Project Start
1983-12-01
Project End
1986-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Thomas Jefferson University
Department
Type
Schools of Medicine
DUNS #
061197161
City
Philadelphia
State
PA
Country
United States
Zip Code
19107
Hawkes, R; Eisenman, L M (1997) Stripes and zones: the origins of regionalization of the adult cerebellum. Perspect Dev Neurobiol 5:95-105
Paradies, M A; Grishkat, H; Smeyne, R J et al. (1996) Correspondence between L7-lacZ-expressing Purkinje cells and labeled olivocerebellar fibers during late embryogenesis in the mouse. J Comp Neurol 374:451-66
Akintunde, A; Eisenman, L M (1994) External cuneocerebellar projection and Purkinje cell zebrin II bands: a direct comparison of parasagittal banding in the mouse cerebellum. J Chem Neuroanat 7:75-86
Eisenman, L M; Hawkes, R (1993) Antigenic compartmentation in the mouse cerebellar cortex: zebrin and HNK-1 reveal a complex, overlapping molecular topography. J Comp Neurol 335:586-605
Eisenman, L M; Schalekamp, M P; Voogd, J (1991) Development of the cerebellar cortical efferent projection: an in-vitro anterograde tracing study in rat brain slices. Brain Res Dev Brain Res 60:261-6
Eisenman, L M; Hawkes, R (1989) 5'-Nucleotidase and the mabQ113 antigen share a common distribution in the cerebellar cortex of the mouse. Neuroscience 31:231-5
Eisenman, L M (1988) Histochemical localization of 5'-nucleotidase in the reeler mutant mouse. Neurosci Lett 94:70-5
Heckroth, J A; Eisenman, L M (1988) The olivocerebellar projection in 'lurcher' mutant mice. Neurosci Lett 85:199-204
Heckroth, J A; Eisenman, L M (1988) Parasagittal organization of mossy fiber collaterals in the cerebellum of the mouse. J Comp Neurol 270:385-94
Blatt, G J; Eisenman, L M (1988) Topographic and zonal organization of the olivocerebellar projection in the reeler mutant mouse. J Comp Neurol 267:603-15

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