Abstract

The long-term objectiveb of this research are to understand developmental mechanisms that are involved during early neurogenesis, particularly those concerned with normal and abnormal closure of the neural tube, as well as with the ongoing processes of proliferation and cytodifferentiation that occur during this crucial period of development.
Specific aims i nclude immunocytochemical localization of neural cell adhesion molecules in dysraphic embryos, a quantitative analysis of neuraxial elongation, manipulation of neural tube elongation and closure in vitro, use of whole embryo culture to determine predictive parameters that are significant in producing the wide range of severity characteristic of lumbosacral dysraphism, and an ultrastructural cytochemical and immunofluorescence analysis of how dysraphism affects early commitment to neural cell lineages. This project will help to contribute to an understanding of the etiology Of specific human neurological disorders such as exencephaly, meningocele, anencephaly, and spina bifida.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS020055-08A1
Application #
3400255
Study Section
Neurology A Study Section (NEUA)
Project Start
1983-12-01
Project End
1995-07-31
Budget Start
1991-08-01
Budget End
1992-07-31
Support Year
8
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
Schools of Medicine
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Wilson, D B; Wyatt, D P (1995) Immunofluorescent analysis of the spinal cord in dysraphic mice. Acta Anat (Basel) 154:120-7
Wilson, D B; Wyatt, D P (1995) Alterations in cranial morphogenesis in the Lp mutant mouse. J Craniofac Genet Dev Biol 15:182-9
Wilson, D B; Wyatt, D P (1995) Patterns of lectin binding during mammalian neurogenesis. J Anat 186 ( Pt 1):209-16
Wilson, D B; Wyatt, D P (1994) Analysis of neurulation in a mouse model for neural dysraphism. Exp Neurol 127:154-8
Wilson, D B; Wyatt, D P (1993) In vitro expression of neural tube pathology in the vl mutant mouse. J Neuropathol Exp Neurol 52:253-9
Wilson, D B; Wyatt, D P (1992) Aberrant convergence of the neural folds in the mouse mutant vl. Teratology 45:105-12
Wilson, D B; Wyatt, D P (1992) Abnormal elevation of the neural folds in the loop-tail mutant mouse. Acta Anat (Basel) 143:89-95
Wilson, D B; Wyatt, D P (1991) Ultrastructural analysis of the midaxial extracellular matrix in spinal dysraphism. Virchows Arch B Cell Pathol Incl Mol Pathol 60:113-8
Wilson, D B; Wyatt, D P; Gookin, J L (1990) Cranial effects of retinoic acid in the loop-tail (Lp) mutant mouse. J Craniofac Genet Dev Biol 10:75-81
Wilson, D B; Wyatt, D P (1989) Axial interactions during neurogenesis in dysraphic mice. Anat Embryol (Berl) 180:515-9

Showing the most recent 10 out of 21 publications