Abstract

The long-term objective of the proposed research is to understand the physiology and pharmacology of the neural systems that transmit and modulate pain.
The specific aim of these studies is to determine the electrical properties of isolated spinothalamic tract cells and the mode of action of a number of transmitter substances on these cells. The objectives of these experiments are (1) to determine the voltage dependent properties of identified spinothalamic cells and (2) to characterize the actions and interactions of peptides and biogenic amines on these cells. The ionic current of identified single STT cells will be measured under voltage clamp conditions. Patch pipette voltage clamp technique will be used. The fundamental knowledge obtained from these experiments will be essential in defining the mechanisms of action of the putative transmitter in spinothalamic cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS023061-03
Application #
3406094
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Project Start
1986-02-01
Project End
1989-03-31
Budget Start
1988-02-01
Budget End
1989-03-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Texas Medical Br Galveston
Department
Type
Schools of Medicine
DUNS #
041367053
City
Galveston
State
TX
Country
United States
Zip Code
77555

  Publications

Xu, G Y; Huang, L Y; Zhao, Z Q (2000) Activation of silent mechanoreceptive cat C and Adelta sensory neurons and their substance P expression following peripheral inflammation. J Physiol 528 Pt 2:339-48
Yajiri, Y; Huang, L Y (2000) Actions of endomorphins on synaptic transmission of Adelta-fibers in spinal cord dorsal horn neurons. J Biomed Sci 7:226-31
Chiou, L C; Huang, L Y (1999) Mechanism underlying increased neuronal activity in the rat ventrolateral periaqueductal grey by a mu-opioid. J Physiol 518 ( Pt 2):551-9
Lai, S L; Gu, Y; Huang, L Y (1998) Dynorphin uses a non-opioid mechanism to potentiate N-methyl-D-aspartate currents in single rat periaqueductal gray neurons. Neurosci Lett 247:115-8
Chen, L; Gu, Y; Huang, L Y (1995) The opioid peptide dynorphin directly blocks NMDA receptor channels in the rat. J Physiol 482 ( Pt 3):575-81
Chen, L; Gu, Y; Huang, L Y (1995) The mechanism of action for the block of NMDA receptor channels by the opioid peptide dynorphin. J Neurosci 15:4602-11
Gu, Y; Huang, L Y (1994) Modulation of glycine affinity for NMDA receptors by extracellular Ca2+ in trigeminal neurons. J Neurosci 14:4561-70
Huang, L M (1992) The excitatory effects of opioids. Neurochem Int 20:463-8
Chen, L; Huang, L Y (1991) Sustained potentiation of NMDA receptor-mediated glutamate responses through activation of protein kinase C by a mu opioid. Neuron 7:319-26
Gu, Y P; Huang, L Y (1991) Block of kainate receptor channels by Ca2+ in isolated spinal trigeminal neurons of rat. Neuron 6:777-84

Showing the most recent 10 out of 15 publications