Abstract

This proposal will focus on the following three subjects: 1) Developmental changes in electrophysiological properties of Xenopus nerve and muscle cells in culture. 2) Synaptic interactions between mammalian clonal cell lines, and 3) Antibody against acetylcholine receptor as a probe for neurospecific interaction. Presynaptic and postsynaptic properties will be sytemmatically examined in Xenopus nerve-muscle cultures. Quantal nature of endplate potentials, spacial distribution of transmitter releasing sites along the length of nerve contact and susceptibility of presynaptic terminals against various treatments will be studied. Postsynaptically, properties of individual acetylcholine receptor (AChR) channels and electrical excitabilities are also planned to be studied. Effort to use clonal cell lines as a model for nerve-muscle interaction will be continued. A system in which both functional transmission and AChR accumulation occur will be searched. Monoclonal antibodies against AChRs will be used as a probe for nerve-muscle interaction. The effect of antibodies against various developmental steps will be examined. If recognition of AChRs were involved in some steps during development, the antibody may dissect out the molecular mechanism of the interaction.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS023753-03
Application #
3407611
Study Section
Neurology B Subcommittee 1 (NEUB)
Project Start
1985-12-01
Project End
1988-11-30
Budget Start
1987-12-01
Budget End
1988-11-30
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Chang, H; Kidokoro, Y (1996) Kinetic properties of glutamate receptor channels in cultured embryonic Drosophila myotubes. Jpn J Physiol 46:249-64
Chang, H; Ciani, S; Kidokoro, Y (1994) Ion permeation properties of the glutamate receptor channel in cultured embryonic Drosophila myotubes. J Physiol 476:1-16
Saito, M; Nguyen, J; Kidokoro, Y (1993) Inhibition of nerve- and agrin-induced acetylcholine receptor clustering on Xenopus muscle cells in culture. Brain Res Dev Brain Res 71:9-17
Kidokoro, Y (1992) Initial uncoordinated expression of three types of voltage-gated currents in cultured Xenopus myocytes. Neurosci Res 13:189-97
Kidokoro, Y; Rohrbough, J (1990) Acetylcholine receptor channels in Xenopus myocyte culture;brief openings, brief closures and slow desensitization. J Physiol 425:227-44
Rohrbough, J; Kidokoro, Y (1990) Changes in kinetics of acetylcholine receptor channels after initial expression in Xenopus myocyte culture. J Physiol 425:245-69
Hirano, Y; Kidokoro, Y (1989) Heparin and heparan sulfate partially inhibit induction of acetylcholine receptor accumulation by nerve in Xenopus culture. J Neurosci 9:1555-61
Kidokoro, Y; Sand, O (1989) Action potentials and sodium inward currents of developing neurons in Xenopus nerve-muscle cultures. Neurosci Res 6:191-208
Kidokoro, Y (1988) Developmental changes in acetylcholine receptor channel properties of vertebrate skeletal muscle. Ion Channels 1:163-82
Kidokoro, Y; Saito, M (1988) Early cross-striation formation in twitching Xenopus myocytes in culture. Proc Natl Acad Sci U S A 85:1978-82

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