The aim of this project is to study the treatment of stroke with hemodilution using basic research techniques. Currently the use of hemodilution with low molecular weight dextran (LMWD) is being evaluated in clinical trials. Hemodilution and hemoconcentration will be evaluated in acute stroke using an animal model of focal cerebral ischemia and the double label autoradiographic technique for determining local cerebral blood flow (LCBF) and local cerebral pH (LCpH) in the same brain section. Data analysis will focus on the measurement of LCBF, LCpH and their ratio in anatomical regions. The border zone of the ischemic area will be evaluated as an area where hemodilution could effect stroke outcome. Hemodilution and hemoconcentration will be used in a chronic model of stroke and evaluated using both neuropathological and autoradiographic criteria. Animals will be studied at various hematocrits to find the optimal hematocrit level. Both plasma and LMWD will be used as hemodilutants to uncover any specific, beneficial effect of LMWD on stroke outcome due to its properties as an inhibitor of red cell and platelet aggregation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
1R01NS024343-01A1
Application #
3408844
Study Section
Neurology A Study Section (NEUA)
Project Start
1987-09-01
Project End
1990-08-31
Budget Start
1987-09-01
Budget End
1988-08-31
Support Year
1
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
017730458
City
Cleveland
State
OH
Country
United States
Zip Code
44195
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Perez-Trepichio, A D; Furlan, A J; Little, J R et al. (1992) Hydroxyethyl starch 200/0.5 reduces infarct volume after embolic stroke in rats. Stroke 23:1782-90;discussion 1790-1
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Salgado, A V; Jones, S C; Furlan, A J et al. (1989) Bimodal treatment with nimodipine and low-molecular-weight dextran for focal cerebral ischemia in the rat. Ann Neurol 26:621-7
Jones, S C; Lu, D F (1988) The evaluation of quantitative autoradiogram processing systems for cerebrovascular research. J Neurosci Methods 24:11-25