Abstract

The purpose of this proposal is to analyze the roles of several monoaminergic systems and the endogenous opioids in the regulation of the secretion of prolactin in male rats. The entire regulating mechanism is highly complex and, therefore, in order to evaluate the role of a specific system in a defined experimental situation it is necessary to delineate not only the influence of that particular system on the secretion of the hormone but also to determine what other systems are involved, what is the contribution of each of them to the overall hormonal response, how they are interconnected, and at what hypothalamic sites, which of the multiple neurotransmitter and opioid receptors are involved and what is the ultimate effector mechanism which leads to a stimulation or inhibition of prolactin secretion. To achieve this goal a variety of neuropharmacological interventions will be used in conjunction with determination of the distribution and metabolism of several monoamines (dopamine, norepinephrine, and serotonin) in microdissected hypothalamic and preoptic areas using high performance liquid chromatography. In some experiments animals with complete deafferentation of the mediobasal hypothalamus will also be used.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS024435-02
Application #
3409053
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Project Start
1988-02-01
Project End
1991-01-31
Budget Start
1989-02-01
Budget End
1990-01-31
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Type
Overall Medical
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390