The phenomenon of dendritic protein synthesis in neurons has been well documented. The specific mRNAs for several proteins with specific dendritic functions or localizations are thought to be targeted to sites of active translation in dendrites. This protein synthesis is associated with the postsynaptic side of synaptic contacts and may provide a mechanism for modulation of postsynaptic functional plasticity. However, the mechanisms for specific transport and targeting of RNA, to dendrites have not been delineated. This application will examine the specific structural elements responsible for localization of BC1, a short, neuron- specific RNA, to dendrites. The specific sequence elements required for targeting to dendrites will be determined and their ability to target foreign chimeric RNA sequences to the dendrite will be evaluated. In the second series of experiments, the role of electrical activity and synaptic transmission on the expression and transport of RNA into dendrites will be assessed. The effects of inhibitors for synaptic transmission, electrical activity, and cytoskeletal organization on various components of the translational apparatus will be examined. It is hoped that these studies will provide new insights into the role of dendritic protein synthesis in neuronal plasticity.
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| Kindler, Stefan; Wang, Huidong; Richter, Dietmar et al. (2005) RNA transport and local control of translation. Annu Rev Cell Dev Biol 21:223-45 |
| Wang, Huidong; Tiedge, Henri (2004) Translational control at the synapse. Neuroscientist 10:456-66 |
| Cristofanilli, Massimiliano; Thanos, Solon; Brosius, Jurgen et al. (2004) Neuronal MAP2 mRNA: species-dependent differential dendritic targeting competence. J Mol Biol 341:927-34 |
| Muslimov, Ilham Aliagaevich; Nimmrich, Volker; Hernandez, Alejandro Ivan et al. (2004) Dendritic transport and localization of protein kinase Mzeta mRNA: implications for molecular memory consolidation. J Biol Chem 279:52613-22 |
| Brosius, Jurgen; Tiedge, Henri (2004) RNomenclature. RNA Biol 1:81-3 |
| Iacoangeli, Anna; Lin, Yuan; Morley, Eric J et al. (2004) BC200 RNA in invasive and preinvasive breast cancer. Carcinogenesis 25:2125-33 |
| Muslimov, Ilham A; Lin, Yuan; Heller, Michal et al. (2002) A small RNA in testis and brain: implications for male germ cell development. J Cell Sci 115:1243-50 |
| Muddashetty, Ravi; Khanam, Tasneem; Kondrashov, Alexander et al. (2002) Poly(A)-binding protein is associated with neuronal BC1 and BC200 ribonucleoprotein particles. J Mol Biol 321:433-45 |
| Muslimov, Ilham A; Titmus, Margaret; Koenig, Edward et al. (2002) Transport of Neuronal BC1 RNA in Mauthner Axons. J Neurosci 22:4293-301 |
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