Abstract

The basic goal of this application is to utilize a hemisection model of the spinal cord in which a gap is created and a mini-guidance channel filled with Schwann cells is inserted. In addition, the model incorporates two additional elements that are intended to maximize the elongation of regenerating axons into the distal cord. These are the administration of methylprednisilone plus the slow release of various neurotrophins via an osmotic mini-pump just caudal to the bridge. Taken together the specific aims of this model could be described as one with a most comprehensive attempt to maximize regeneration into the host cord tissue caudal to a Schwann cell bridged lesion.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
3R01NS036350-03S1
Application #
6322282
Study Section
Neurology B Subcommittee 2 (NEUB)
Program Officer
Chiu, Arlene Y
Project Start
1998-05-15
Project End
2001-05-31
Budget Start
2000-05-01
Budget End
2001-05-31
Support Year
3
Fiscal Year
2000
Total Cost
$40,000
Indirect Cost

  Institution

Name
Saint Louis University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
City
Saint Louis
State
MO
Country
United States
Zip Code
63103

  Publications

Walker, Melissa J; Walker, Chandler L; Zhang, Y Ping et al. (2015) A novel vertebral stabilization method for producing contusive spinal cord injury. J Vis Exp :e50149
Walker, Chandler L; Liu, Nai-Kui; Xu, Xiao-Ming (2013) PTEN/PI3K and MAPK signaling in protection and pathology following CNS injuries. Front Biol (Beijing) 8:
Hu, Jian-Guo; Wang, Xiao-Fei; Deng, Ling-Xiao et al. (2013) Cotransplantation of glial restricted precursor cells and Schwann cells promotes functional recovery after spinal cord injury. Cell Transplant 22:2219-36
Deng, Ling-Xiao; Deng, Ping; Ruan, Yiwen et al. (2013) A novel growth-promoting pathway formed by GDNF-overexpressing Schwann cells promotes propriospinal axonal regeneration, synapse formation, and partial recovery of function after spinal cord injury. J Neurosci 33:5655-67
Walker, Chandler L; Walker, Melissa J; Liu, Nai-Kui et al. (2012) Systemic bisperoxovanadium activates Akt/mTOR, reduces autophagy, and enhances recovery following cervical spinal cord injury. PLoS One 7:e30012
Shi, Yunzhou; Zhang, Delong; Huff, Terry B et al. (2011) Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord. J Biomed Opt 16:106012
Deng, Ling-Xiao; Hu, Jianguo; Liu, Naikui et al. (2011) GDNF modifies reactive astrogliosis allowing robust axonal regeneration through Schwann cell-seeded guidance channels after spinal cord injury. Exp Neurol 229:238-50
Wang, Xiaofei; Smith, George M; Xu, Xiao-Ming (2011) Preferential and bidirectional labeling of the rubrospinal tract with adenovirus-GFP for monitoring normal and injured axons. J Neurotrauma 28:635-47
Zou, Jian; Wang, Yan-Xia; Dou, Fang-Fang et al. (2010) Glutamine synthetase down-regulation reduces astrocyte protection against glutamate excitotoxicity to neurons. Neurochem Int 56:577-84
Zhang, Liqun; Ma, Zhengwen; Smith, George M et al. (2009) GDNF-enhanced axonal regeneration and myelination following spinal cord injury is mediated by primary effects on neurons. Glia 57:1178-91

Showing the most recent 10 out of 25 publications