This is an anatomical project to study the organization of visceral pathways to the forebrain. These pathways are hypothesized to mediate viscerally induced changes in emotion, cortical arousal, and behavior. The specific hypothesis to be tested is that visceral and especially cardiopulmonary stimuli, reach the cerebral cortex through projections from the nucleus of the solitary (NTS) tract to midline-intralaminar thalamic nuclei (MIT), where they may be widely distributed to cortex and basal ganglia. Study I will use anterograde and retrograde tracers to determine: a) whether there is a direct MIT connection from cardiopulmonary afferents; b) if so, whether the projection arises from specific subnuclei within the NTS; c) if the MIT inputs are topographically organized; d) if the NTS-MIT connection is characterized by a particular transmitter phenotype; e) if, using electron microscopy, the NTS-MIT projection neurons are directly innervated by primary vagal fibers. Other studies will determine the extent to which the indirect NTS pathway from the rostral ventrolateral medulla (RVL) is connected with MIT, focusing on known functional subdivisions of the RVL. Study II will determine the cortical and basal ganglia targets of visceroreceptive MIT projection neurons. Study III will establish the extent to which the visceral projections to the MIT are collateralized to provide parallel processing to other areas known to process emotional and autonomic information, such as the RVL, intermediolateral cell column, paraventricular nucleus of the hypothalamus, and amygdala. Study IV will examine, by measuring expression of c-fos and c-jun, whether RVL or vagal stimulation, or changes in arterial pressure, will activate neurons within these circuits.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
3R01NS036363-03S1
Application #
6076463
Study Section
Neurology B Subcommittee 2 (NEUB)
Program Officer
Kitt, Cheryl A
Project Start
1997-05-01
Project End
2001-02-28
Budget Start
1998-03-01
Budget End
1999-02-28
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Psychiatry
Type
Schools of Medicine
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032
Welch, M G; Keune, J D; Welch-Horan, T B et al. (2004) Secretin: hypothalamic distribution and hypothesized neuroregulatory role in autism. Cell Mol Neurobiol 24:219-41
Burke, William J; Li, Shu Wen; Chung, Hyung D et al. (2004) Neurotoxicity of MAO metabolites of catecholamine neurotransmitters: role in neurodegenerative diseases. Neurotoxicology 25:101-15
Welch, Martha G; Keune, Jason D; Welch-Horan, T Bramwell et al. (2003) Secretin activates visceral brain regions in the rat including areas abnormal in autism. Cell Mol Neurobiol 23:817-37
Burke, W J; Li, S W; Zahm, D S et al. (2001) Catecholamine monoamine oxidase a metabolite in adrenergic neurons is cytotoxic in vivo. Brain Res 891:218-27
Golanov, E V; Ruggiero, D A; Reis, D J (2000) A brainstem area mediating cerebrovascular and EEG responses to hypoxic excitation of rostral ventrolateral medulla in rat. J Physiol 529 Pt 2:413-29
Ruggiero, D A; Underwood, M D; Mann, J J et al. (2000) The human nucleus of the solitary tract: visceral pathways revealed with an ""in vitro"" postmortem tracing method. J Auton Nerv Syst 79:181-90
Sica, A L; Greenberg, H E; Scharf, S M et al. (2000) Immediate-early gene expression in cerebral cortex following exposure to chronic-intermittent hypoxia. Brain Res 870:204-10
Sica, A L; Greenberg, H E; Scharf, S M et al. (2000) Chronic-intermittent hypoxia induces immediate early gene expression in the midline thalamus and epithalamus. Brain Res 883:224-8
Ruggiero, D A; Gootman, P M; Ingenito, S et al. (1999) The area postrema of newborn swine is activated by hypercapnia: relevance to sudden infant death syndrome? J Auton Nerv Syst 76:167-75
Sica, A L; Gootman, P M; Ruggiero, D A (1999) CO(2)-induced expression of c-fos in the nucleus of the solitary tract and the area postrema of developing swine. Brain Res 837:106-16

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