Several human neuromuscular disorders which present as skeletal muscle weakness are associated with presynaptic impairment of release of acetycholine (ACh). These include botulism, envenomation by certain poisonous snakes and arthropods as well as several human congenital myasthenic syndromes. The best characterized of the presynaptic neuromuscular disorders is Lambert-Eaton Myasthenic Syndrome (LEMS) which often associates with oat cell carcinoma. Skeletal muscle weakness in LEMS is thought to result from autoimmune attack on the Ca channel functional complex at the motor nerve terminal. The objective of this project is to study animal models with disruptions of motor nerve terminal ACh release to learn more about the etiology of diseases such as LEMS. Multiple subtypes of Ca channels such as the N-, P/Q-, R- and L-type exist in motor nerve terminals. P/Q-type Ca channels are primarily responsible for controlling ACh release at mammalian neuromuscular junctions and hence are the presumed primary autoimmune target in LEMS. Autoantibodies are thought to target primarily the a1 subunit of the channel, although other proteins have also been postulated as antigenic targets in LEMS. In the passive transfer mode of LEMS in mice, the phenotype of Ca channel controlling ACh release from motor terminals changes, and expresses a significant L-type component, suggesting a compensatory change occurs to make up for functional loss of the normal P/Q complement of Ca channels. In this project, intracellular microelectrode recordings of synaptic potentials, perineurial recordings of nerve terminal Ca channel activity, recordings of FM1-43 fluorescence and nerve terminal Ca channel subunit immunocytochemistry will be used to study neuromuscular transmission following passive transfer of LEMS to mice in which the a1a subunit of the P/Q-type of Ca channel is genetically-altered, either by deletion or point mutation, or in which the normal subunit which coexpresses with the P/Q-type Ca channel CA subunit is mutated.
The aims are: I. Can LEMS be passively transferred to genetically-altered mice lacking functional or having mutated P/Q-type Ca channels and do they respond differently than do wildtype mice? Do these mice express L-type Ca channel activity normally, and is it altered after passive transfer of LEMS? Do L-type channels play a greater role in regulating ACh release in motor nerve terminals of mice missing the a1a gene of the P/Q- type Ca channel or in which mutations such as """"""""tottering"""""""" or """"""""leaner"""""""" occur in the a1a subunit? Do L-type Ca channels become present or unmasked under either of these conditions? 2. Which Ca channels are involved in nerve-evoked release of ACh from nerve terminals of animals lacking P/Q-type Ca channels, or having mutations in the pore-forming region. 3. What role does the Ca channel 0 subunit play in ACh release at neuromuscular junctions? Will animals having a mutation (""""""""lethargic"""""""") in the 04 subunit, be susceptible to induction of LEMS? Results of this study should provide a better understanding of the etiology of LEMS and subsequent changes that occur at motor nerve terminals in patients with LEMS. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
1R01NS051833-01A1
Application #
7029507
Study Section
Clinical Neuroplasticity and Neurotransmitters Study Section (CNNT)
Program Officer
Porter, John D
Project Start
2006-01-01
Project End
2009-12-31
Budget Start
2006-01-01
Budget End
2006-12-31
Support Year
1
Fiscal Year
2006
Total Cost
$339,750
Indirect Cost
Name
Michigan State University
Department
Pharmacology
Type
Schools of Veterinary Medicine
DUNS #
193247145
City
East Lansing
State
MI
Country
United States
Zip Code
48824
Hajela, Ravindra K; Huntoon, Kristin M; Atchison, William D (2015) Lambert-Eaton syndrome antibodies target multiple subunits of voltage-gated Ca2+ channels. Muscle Nerve 51:176-84
Molina-Campos, Elizabeth; Xu, Youfen; Atchison, William D (2015) Age-dependent contribution of P/Q- and R-type Ca2+ channels to neuromuscular transmission in lethargic mice. J Pharmacol Exp Ther 352:395-404
Pardo, Nicole E; Hajela, Ravindra K; Atchison, William D (2006) Acetylcholine release at neuromuscular junctions of adult tottering mice is controlled by N-(cav2.2) and R-type (cav2.3) but not L-type (cav1.2) Ca2+ channels. J Pharmacol Exp Ther 319:1009-20