Due to unprecedented increases in the elderly populations, age-related diseases are expected to increase exponentially in the coming years. Among these, neurodegenerative diseases will be the most devastating in terms of their emotional and economic effects. In the next decade, at least 2% of Americans will be afflicted with some form of Alzheimer's disease (AD) (4,000,000), Parkinson's disease (PD) (1,500,000), or Huntington's disease (HD) (200,000), among others. Each of these disorders can affect patients for decades, yet no effective long-term approaches to therapy are currently available. In this proposal, we aim to test mechanisms that prevent or delay onset of and progression of HD. We have surprisingly found that a DNA oxidative damage causes the somatic expansion in Huntington's Disease that is observed with age and governs onset that begins around mid-life. Loss of a single base excision repair enzyme, 8-oxo-guanine glycosylase (OGG1), in hHD/OGG1(-/-) mice stops age-dependent expansion of CAG triplet repeats that is observed in mhtt mice. A second surprise was that loss of expansion was accompanied by an unforeseen amelioration (or at least a substantial delay) of pathophysiology. Animals expressing hHD protein but lacking OGG1 appeared devoid of visible signs of disease typically observed late in the lifetime of these animals. These data demonstrate, for the first time, the unexpected finding that oxidation of DNA bases and/or their removal are causative factors in both DNA expansion and the onset of toxicity. Our discovery that loss of OGG1 stops expansion provides a direct molecular link between oxidative DNA damage and the mechanism of expansion, and onset of pathophysiology. We have created a new HD mouse model in which an mhttexpressing animal of 150 repeats (HD150KI) is crossed with an animals lacking OGG1(-/-) producing (HD150KI/OGG1(-/-)) progeny. HD150KI animals display a neurological phenotype, transmit an expanded allele, and expand the inherited allele in somatic tissues with age. In contrast, HD150KI/OGG1(-/-) animals can inherit the CAG repeat but cannot expand their repeats in somatic brain cells. Both mice express mhtt. Since, the OGG1(-/-) animals have no observable neurological phenotype on their own, the HD150 KI/OGG1(-/-) mice will allow us to test whether loss of somatic expansion alters disease onset relative to their mhtt-expressing counterparts (HD150KI). Our model predicts that suppressing somatic expansion will offset or delay the onset of motor dysfunction and other measures of disease pathophysiology in HD150 KI/OGG1(-/-) mice relative to HD150KI animals. We will also test whether OGG1 activity is the source of both the somatic expansion and rise in the peripheral biomarkers associated with disease onset. We will test whether reduction in somatic expansion observed in HD150 KI/OGG1(-/-) mice is accompanied by reduction in the biomarker relative to HD150KI animals. In a reverse approach, we will reduce biomarker production in HD150KI mice by treatment with anti-oxidants, and test whether somatic expansion is also reduced.

Public Health Relevance

The mutant Huntington's Disease (HD) protein (mhtt) is an inherited, neurodegenerative disorder in which there is progressive worsening of the symptoms with age. Toxicity arises from an expanded polyglutamine segments in the mutant protein that is encoded from a CAG repeat tract within the human HD gene. The longer the CAG tract is the more severe the phenotype. Although HD is an inherited disease, recent data have suggested that disease progression may not be governed entirely by the inherited allele. We demonstrate that onset of disease and its progression may be regulated by somatic growth of the CAG repeats that occurs in the brain with age. The age-dependent somatic mutation occurs in the process of removing oxidized base lesions, and is dependent on a single base excision repair enzyme, 7,8-dihydro-8-oxo-guanine-DNA glycosylase (OGG1). Loss of OGG1 in animals mhtt-expressing animals not only suppresses CAG expansion in mhtt- expressing animals, but preliminary results indicate that suppression of expansion is also accompanied by an apparent amelioration (or at least a substantial delay) of pathophysiology. If OGG1 is a common cause of somatic expansion then inhibiting OGG1 activity may be beneficial as a therapeutic intervention to stop or delay disease progression.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS062384-02
Application #
7915815
Study Section
Neural Oxidative Metabolism and Death Study Section (NOMD)
Program Officer
Sutherland, Margaret L
Project Start
2009-09-01
Project End
2012-08-31
Budget Start
2010-09-01
Budget End
2012-08-31
Support Year
2
Fiscal Year
2010
Total Cost
$642,465
Indirect Cost
Name
Lawrence Berkeley National Laboratory
Department
Neurosciences
Type
Organized Research Units
DUNS #
078576738
City
Berkeley
State
CA
Country
United States
Zip Code
94720
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Platt, Virginia; Lee, Do Yup; Canaria, Christie A et al. (2013) Towards understanding region-specificity of triplet repeat diseases: coupled immunohistology and mass spectrometry imaging. Methods Mol Biol 1010:213-30
Lee, Do Yup; Xun, Zhiyin; Platt, Virginia et al. (2013) Distinct pools of non-glycolytic substrates differentiate brain regions and prime region-specific responses of mitochondria. PLoS One 8:e68831
Budworth, Helen; McMurray, Cynthia T (2013) Bidirectional transcription of trinucleotide repeats: roles for excision repair. DNA Repair (Amst) 12:672-84
Budworth, Helen; McMurray, Cynthia T (2013) A brief history of triplet repeat diseases. Methods Mol Biol 1010:3-17
Xun, Zhiyin; Rivera-Sánchez, Sulay; Ayala-Peña, Sylvette et al. (2012) Targeting of XJB-5-131 to mitochondria suppresses oxidative DNA damage and motor decline in a mouse model of Huntington's disease. Cell Rep 2:1137-42
Majka, Jerzy; Alford, Brian; Ausio, Juan et al. (2012) ATP hydrolysis by RAD50 protein switches MRE11 enzyme from endonuclease to exonuclease. J Biol Chem 287:2328-41
Lee, Do Yup; Platt, Virginia; Bowen, Ben et al. (2012) Resolving brain regions using nanostructure initiator mass spectrometry imaging of phospholipids. Integr Biol (Camb) 4:693-9
Xun, Zhiyin; Lee, Do-Yup; Lim, James et al. (2012) Retinoic acid-induced differentiation increases the rate of oxygen consumption and enhances the spare respiratory capacity of mitochondria in SH-SY5Y cells. Mech Ageing Dev 133:176-85
Lang, Walter H; Coats, Julie E; Majka, Jerzy et al. (2011) Conformational trapping of mismatch recognition complex MSH2/MSH3 on repair-resistant DNA loops. Proc Natl Acad Sci U S A 108:E837-44

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