The cerebellum is critical for learning and executing coordinated, well-timed movements. The cerebellar cortex seems to have a particular role in learning to time movements. Since the 1960's and 70's, we have known the architecture of the cerebellar microcircuit, but most analyses of cerebellar function during behavior have focused on Purkinje cells. Here, we propose to investigate the cerebellar cortex at an entirely new level by asking how the full cerebellar microcircuit ? mossy fiber, granule cells, Golgi cells, molecular layer interneurons, and Purkinje cells ? performs neural computations during motor behavior and motor learning. We strive to ?crack? the circuit by identifying all elements, recording their electrical activity during movement and learning, and reconstructing a neural circuit model that reproduces the biological data. We will use three established learning systems that all can learn predictive timing: classical conditioning of the eyelid response (mice), predictive timing of forelimb movements (mice), and direction learning in smooth pursuit eye movements (monkeys). Our proposal has six key features. First, optogenetics (in mice) will link the discharge of different cerebellar interneurons during movement and learning to their molecular cell types. Second, a machine-learning clustering analysis (in mice and monkeys) will find analogies among the cell populations recorded in our three preparations and will classify neurons according to their putative cell types based on recordings of many parameters of non-Purkinje cells during movement and motor learning. Third, multi- contact electrodes will allow us to record simultaneously from multiple neighboring single neurons and compute spike-timing cross-correlograms (CCGs) to identify the sign of connections; we also will look for changes in CCGs that provide evidence of specific sites of plasticity during learning. Fourth, gCAMP imaging of the granule cell layer will reveal the temporal structure of inputs to the cerebellar microcircuit, and determine whether those inputs are modified in relation to motor learning. Fifth, a model neural network with realistic cerebellar architecture will reveal a single set of model parameters that will transform the measured inputs to the cerebellum in our three movement systems to the measured responses of all neurons in the cerebellar cortex. Sixth, the model will elucidate how mechanisms of synaptic and cellular plasticity at different sites in the cerebellar microcircuit work together to cause motor learning.

Public Health Relevance

The cerebellum is critical for learning and executing coordinated and well-timed movements. A better understanding of how the cerebellar circuit works and learns will be important for improving human health in cases of stroke, neuro-degenerative motor disease, developmental motor disorders, and adult movement disorders.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS112917-02
Application #
9976609
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
David, Karen Kate
Project Start
2019-08-01
Project End
2024-04-30
Budget Start
2020-05-01
Budget End
2021-04-30
Support Year
2
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Duke University
Department
Neurosciences
Type
Schools of Medicine
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705