. The potential usefulness and importance of topical in-vivo ESR are cited as the following unique properties which distinguish it from other techniques: a) In-vivo detection of extrinsic paramagnetic species (spin labels) targeted to specific sites in a biological system. b) In vivo monitoring using spin labels which monitor structural order at the microscopic level (membranes, proteins, cell walls, etc.) c) Direct in-vivo detection of free radical species (naturally occurring or """"""""trapped"""""""" products from transient free radical reactions).
The specific aims of this Research Resource development renewal proposal are: 1. Further development and optimization of low field in-vivo topical ESR spectrometers, including microwave resonators in the low GHz range (500-1000 MHz); assessment of feasibility, sensitivity, power losses, and specifications of a low field topical ESR system. 2. In-vivo monitoring of spin label distribution and metabolism in animals: a. in-vivo pharmacokinetics of nitroxide metabolism and excretion b. monitoring tumour growth characteristics by spin label metabolism c. spin trapping studies of a swine global myocardial ischemia reperfusion model; d. nitroxide monitoring in the brain.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
2R01RR003126-04
Application #
3421466
Study Section
Special Emphasis Panel (SSS (C))
Project Start
1987-09-15
Project End
1994-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
4
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Ohio State University
Department
Type
Schools of Arts and Sciences
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210