Abstract

The purpose of the present proposal is to develop and describe syngeneic (isogenic) strains of rainbow trout as a lower vertebrate aquatic animal model for biomedical research. In particular, we will develop these fish for the study of immunological phenomena. These trout will be useful not only for immunological research such as immunogenetics and neuroimmunology, but will fill many niches in such diverse areas as endocrinology, developmental biology, aquatic toxicology, gene transfer technology and carcinogenesis research.
The specific aims of the project are the propagation of existing and development of new isogenic lines by androgenesis and gynogenesis, validation of the isogenic nature of the derived fish; and further development of immunological techniques using the trout as models.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
5R01RR006654-04
Application #
3421683
Study Section
Special Emphasis Panel (SRC)
Project Start
1990-09-30
Project End
1995-09-29
Budget Start
1993-09-30
Budget End
1994-09-29
Support Year
4
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Washington State University
Department
Type
Other Domestic Higher Education
DUNS #
041485301
City
Pullman
State
WA
Country
United States
Zip Code
99164

  Publications

Martinez, Victor; Thorgaard, Gary; Robison, Barrie et al. (2005) An application of Bayesian QTL mapping to early development in double haploid lines of rainbow trout including environmental effects. Genet Res 86:209-21
Zhang, Hui; Thorgaard, Gary H; Ristow, Sandra S (2002) Molecular cloning and genomic structure of an interleukin-8 receptor-like gene from homozygous clones of rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 13:251-8
Robison, B D; Wheeler, P A; Sundin, K et al. (2001) Composite interval mapping reveals a major locus influencing embryonic development rate in rainbow trout (Oncorhynchus mykiss). J Hered 92:16-22
Zhang, H; Nichols, K; Thorgaard, G H et al. (2001) Identification, mapping, and genomic structural analysis of an immunoreceptor tyrosine-based inhibition motif-bearing C-type lectin from homozygous clones of rainbow trout (Oncorhynchus mykiss). Immunogenetics 53:751-9
Brunelli, J P; Robison, B D; Thorgaard, G H (2001) Ancient and recent duplications of the rainbow trout Wilms' tumor gene. Genome 44:455-62
Zhang, H; Evenhuis, J P; Thorgaard, G H et al. (2001) Cloning, characterization and genomic structure of the natural killer cell enhancement factor (NKEF)-like gene from homozygous clones of rainbow trout (Oncorhynchus mykiss). Dev Comp Immunol 25:25-35
Zhang, H; Robison, B; Thorgaard, G H et al. (2000) Cloning, mapping and genomic organization of a fish C-type lectin gene from homozygous clones of rainbow trout (Oncorhynchus mykiss). Biochim Biophys Acta 1494:14-22
Ristow, S S; Grabowski, L D; Thompson, S M et al. (1999) Coding sequences of the MHC II beta chain of homozygous rainbow trout (Oncorhynchus mykiss). Dev Comp Immunol 23:51-60
Brunelli, J P; Thorgaard, G H (1999) Sequence, expression and genetic mapping of a rainbow trout retinoblastoma cDNA. Gene 226:175-80
Young, W P; Wheeler, P A; Coryell, V H et al. (1998) A detailed linkage map of rainbow trout produced using doubled haploids. Genetics 148:839-50

Showing the most recent 10 out of 17 publications