The project is aimed at understanding how to enhance two-photon absorption cross-sections of organic molecules to develop two-photon fluorophores. This could greatly enhance the applicability of two-photon fluorescence scanning microscopy (TPFSM). Guidelines for the synthesis of suitable molecules include high two-photon absorption cross section, high fluorescence quantum yield, tunable hydrophilicity, incorporation of functional groups for selective labeling, and photochemical stability to prevent rapid bleaching under laser irradiation.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
5R01RR014342-02
Application #
6188809
Study Section
Biophysical Chemistry Study Section (BBCB)
Program Officer
Swain, Amy L
Project Start
1999-08-01
Project End
2002-07-31
Budget Start
2000-08-01
Budget End
2001-07-31
Support Year
2
Fiscal Year
2000
Total Cost
$205,275
Indirect Cost
Name
University of Arizona
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
City
Tucson
State
AZ
Country
United States
Zip Code
85721