BP1 is a member of homeobox genes, which are a family of regulatory genes encoding transcription factors that are vital for all aspects of growth and differentiation. The deregulated expression of homeobox genes has been described in many solid tumors and derivative cell lines. Among these, HOX, HSIX1 and PAX have been shown to promote cellular transformation in breast cancer. Recent studies demonstrate that homeobox genes impinge on the cell cycle, indicating that deregulation of cell cycle control might be a common mechanism by which aberrant homeobox gone expression contributes to carcinogenesis. This application will focus on the role of deregulated BP1 expression in cell cycle control in breast carcinogenesis. The oncogenic potential of BP1 will be established using clonogenicity and anchorage-independent growth studies by overexpressing BP1 in normal or Iow-BP1 expressing mammary cell lines. The downstream BP1 targets will be discovered using a microarray approach. Furthermore, the potential regulatory pathway of BP1 during cell cycle progression will be elucidated. These studies would potentially establish BP1 as a biomarker in breast cancer diagnosis and treatment.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Research Grants (R03)
Project #
1R03CA102928-01A1
Application #
6831113
Study Section
Special Emphasis Panel (ZCA1-SRRB-Q (M1))
Program Officer
Wang, Wendy
Project Start
2004-08-01
Project End
2006-07-31
Budget Start
2004-08-01
Budget End
2005-07-31
Support Year
1
Fiscal Year
2004
Total Cost
$76,500
Indirect Cost
Name
George Washington University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
043990498
City
Washington
State
DC
Country
United States
Zip Code
20052
Fu, Sidney W; Chen, Liang; Man, Yan-Gao (2011) miRNA Biomarkers in Breast Cancer Detection and Management. J Cancer 2:116-22
Kluk, Brian J; Fu, Yebo; Formolo, Trina A et al. (2010) BP1, an isoform of DLX4 homeoprotein, negatively regulates BRCA1 in sporadic breast cancer. Int J Biol Sci 6:513-24
Song, Yongchun; Dang, Chengxue; Fu, Yebo et al. (2009) Genome-wide analysis of BP1 transcriptional targets in breast cancer cell line Hs578T. Int J Biol Sci 5:1-12
Stevenson, Holly S; Fu, Sidney W; Pinzone, Joseph J et al. (2007) BP1 transcriptionally activates bcl-2 and inhibits TNFalpha-induced cell death in MCF7 breast cancer cells. Breast Cancer Res 9:R60