Abstract

This application proposes to test the hypothesis that reduction of oral tumor cell EGF receptor mRNA can lead to inhibition of tumor cell growth. There are two specific aims proposed to test the stated hypothesis.
Aim 1 proposes to construct an inducible and stable vector for ribozyme expression.
Aim 2 proposes to test ribozyme inhibition of EGFR expression at the RNA and protein levels and to test their effects on the transformed phenotype of oral cancer cells which overexpress EGFR.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Small Research Grants (R03)
Project #
5R03DE011869-02
Application #
2414708
Study Section
NIDCR Special Grants Review Committee (DSR)
Project Start
1996-05-01
Project End
1998-04-30
Budget Start
1997-05-01
Budget End
1998-04-30
Support Year
2
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center Houston
Department
Other Basic Sciences
Type
Schools of Dentistry
DUNS #
City
Houston
State
TX
Country
United States
Zip Code
77225

  Publications

Chen, Z; Ke, L D; Yuan, X H et al. (2000) Correlation of cisplatin sensitivity with differential alteration of EGFR expression in head and neck cancer cells. Anticancer Res 20:899-902
Arany, I; Adler-Storthz, K; Chen, Z et al. (1999) Local inflammation may influence oral tumor cell differentiation. Anticancer Res 19:1065-7
Brysk, M M; Lei, G; Adler-Storthz, K et al. (1999) Zinc-alpha2-glycoprotein expression as a marker of differentiation in human oral tumors. Cancer Lett 137:117-20
Arany, I; Adler-Storthz, K; Chen, Z et al. (1998) Tumor differentiation-dependent local immunity in human head and neck cancers. Cancer Lett 123:173-6
Ke, L D; Adler-Storthz, K; Clayman, G L et al. (1998) Differential expression of epidermal growth factor receptor in human head and neck cancers. Head Neck 20:320-7
Brysk, M M; Lei, G; Adler-Storthz, K et al. (1998) Differentiation and cathepsin D expression in human oral tumors. Laryngoscope 108:1234-7
Arany, I; Adler-Storthz, K; Chen, Z et al. (1997) Differentiation markers in oral carcinoma cell lines and tumors. Anticancer Res 17:4607-10